Difference between revisions of "Part:BBa K5207010"

 
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This is an expression cassette consisting of a promoter pTDH3, the SmGGPPS gene, and a terminator tENO1.
 
This is an expression cassette consisting of a promoter pTDH3, the SmGGPPS gene, and a terminator tENO1.
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5207010 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5207010 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K5207010 parameters</partinfo>
 
<partinfo>BBa_K5207010 parameters</partinfo>
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===Usage and Biology===
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We combined each Level 0 junction module, promoter, CDS, terminator, and ending module, and constructed them into vector pYTK095 through BsaI digestion and ligation, and this step was the construction of Level 1.
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<html>
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<body>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.wiki/teams/5207/parts/4.jpg" style = "width:600px"></center>
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</div>
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<figcaption><center>Figure 1. Snapgene diagrams of pYTK095</center></figcaption>
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</figure>
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</body>
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</html>
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After transformation in E. coli DH10B, we selected the single bacterial colony (pYTK095) that does not fluoresce under UV light from the Petri dishes. It was used for colony PCR amplification and validation.
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<html>
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<body>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.wiki/teams/5207/parts/5.jpg" style = "width:600px"></center>
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</div>
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<figcaption><center>Figure 2. Fluorescent labeling screening of pYTK095</center></figcaption>
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</figure>
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</body>
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</html>
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<html>
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<body>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.wiki/teams/5207/parts/6.jpg" style = "width:600px"></center>
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</div>
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<figcaption><center>Figure 3. Validation plot of pYTK095 gel electrophoresis</center></figcaption>
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</figure>
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</body>
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</html>
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Revision as of 09:03, 30 September 2024


pTDH3-SmGGPPS-tENO1

This is an expression cassette consisting of a promoter pTDH3, the SmGGPPS gene, and a terminator tENO1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 685
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 861
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

We combined each Level 0 junction module, promoter, CDS, terminator, and ending module, and constructed them into vector pYTK095 through BsaI digestion and ligation, and this step was the construction of Level 1.

Figure 1. Snapgene diagrams of pYTK095

After transformation in E. coli DH10B, we selected the single bacterial colony (pYTK095) that does not fluoresce under UV light from the Petri dishes. It was used for colony PCR amplification and validation.

Figure 2. Fluorescent labeling screening of pYTK095

Figure 3. Validation plot of pYTK095 gel electrophoresis