Difference between revisions of "Part:BBa K5079015"
| Line 3: | Line 3: | ||
<partinfo>BBa_K5079015 short</partinfo> | <partinfo>BBa_K5079015 short</partinfo> | ||
| − | + | This is the forward primer for Part:BBa_K5079033. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: ACTTTAAGAAGGAGATATAC atgagcggcaacccattag. 3’ GC Content is 53%, 3' length is 19nt and 3' Tm is 65°C. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 09:01, 30 September 2024
K45_CP_N114 Switch primers into pET-28a Switch 8 (Fwd)
This is the forward primer for Part:BBa_K5079033. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: ACTTTAAGAAGGAGATATAC atgagcggcaacccattag. 3’ GC Content is 53%, 3' length is 19nt and 3' Tm is 65°C.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]