Difference between revisions of "Part:BBa K206000:Design"

(→‎Design Notes)
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__NOTOC__
 
__NOTOC__
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<partinfo>BBa_K206000 short</partinfo>
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{|
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|<div style="align: center; valign: center; font-family: Arial; font-size: 12pt">
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{|
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|-
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|width="60px"|''Name'':
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|pBAD strong
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|-
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|width="60px"|''Input'':
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|width="100px"|[http://openwetware.org/wiki/Arabinose L-arabinose]
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|-
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|''Output'':
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| PoPS
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|}
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</div>
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<hr width="800px">
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<div class="noprint" style="padding: 10px; color: #ffffff; background-color: #C0C0C0; width: 800px; align: center">
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<center>
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[[Part:BBa_K206000 |Part Main Page]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Part:BBa_K206000:Design |Part Design]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Part:BBa_K206000:Characterization |Characterization]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[pBAD Promoter Family |Family]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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[[Part:BBa_K206000:Experience |Add Data]] &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;
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</center>
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</div>
  
 
<partinfo>BBa_K206000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K206000 SequenceAndFeatures</partinfo>
  
  
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===Design Notes===
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==Design Notes==
 
Niland et al. found that certain mutations in the AraI1 site increased binding of the DNA to the AraC protein. We applied all of these mutations in an attempt to create a stronger version of the pBAD promoter.
 
Niland et al. found that certain mutations in the AraI1 site increased binding of the DNA to the AraC protein. We applied all of these mutations in an attempt to create a stronger version of the pBAD promoter.
  
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===Source===
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==Source==
 
Site-directed mutagenesis was performed on <partinfo>I13453</partinfo> using the following primers:<br>
 
Site-directed mutagenesis was performed on <partinfo>I13453</partinfo> using the following primers:<br>
 
Forward: TAATCTTATGGACTATCTTGCTATGGCATAGC<br>
 
Forward: TAATCTTATGGACTATCTTGCTATGGCATAGC<br>
 
Reverse: GCGGATCCTACCTGACGCTTTTTATC
 
Reverse: GCGGATCCTACCTGACGCTTTTTATC
  
−
===References===
+
==References==
 
Niland P, Hühne R, Müller-Hill B. (1996). How AraC Interacts Specifically with its Target DNAs. ''J. Mol. Biol.'' 264:667-674. [http://www.ncbi.nlm.nih.gov/pubmed/8980677]
 
Niland P, Hühne R, Müller-Hill B. (1996). How AraC Interacts Specifically with its Target DNAs. ''J. Mol. Biol.'' 264:667-674. [http://www.ncbi.nlm.nih.gov/pubmed/8980677]

Revision as of 23:15, 21 October 2009


Name: pBAD strong
Input: [http://openwetware.org/wiki/Arabinose L-arabinose]
Output: PoPS

Part Main Page        Part Design        Characterization        Family        Add Data       


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Niland et al. found that certain mutations in the AraI1 site increased binding of the DNA to the AraC protein. We applied all of these mutations in an attempt to create a stronger version of the pBAD promoter.

Source

Site-directed mutagenesis was performed on BBa_I13453 using the following primers:
Forward: TAATCTTATGGACTATCTTGCTATGGCATAGC
Reverse: GCGGATCCTACCTGACGCTTTTTATC

References

Niland P, Hühne R, Müller-Hill B. (1996). How AraC Interacts Specifically with its Target DNAs. J. Mol. Biol. 264:667-674. [http://www.ncbi.nlm.nih.gov/pubmed/8980677]