Difference between revisions of "Part:BBa K5107004"

 
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<partinfo>BBa_K5107004 short</partinfo>
 
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ERE5 is the five tandem repeats of the ERE minimal:[https://parts.igem.org/Part:BBa_K5107001 BBa_K5107001] to increase the interaction surface of the hormone receptor with the DNA.
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===Usage and Biology===
 
===Usage and Biology===
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Previously, in-cell systems have successfully used five tandem repeats of the REs for different human hormonal receptors<html><a href="#ref1">[1]</a></html>.
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Inserted in plasmids such as those we used for our in cell-assay, it is shown efficient to express Lac-Z when the associated hormone-receptor complex is present in the cell. In this manner, hormone work as trancription activator.
  
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<span class='h3bb'>Sequence and Features</span>
 
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        <img src="https://static.igem.wiki/teams/5107/eng-incellsystem.webp"width="500" height="500">
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        <figcaption><b>Figure 1:</b>Schematic overview of the in cell system which uses the Lac-Z as reporter gene when hormone/edc i spresent in the cell.</figcaption>
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===Sequence and Features===
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<partinfo>BBa_K5107004 SequenceandFeatures</partinfo>
  
 
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<partinfo>BBa_K5107004 parameters</partinfo>
 
<partinfo>BBa_K5107004 parameters</partinfo>
 
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===References===
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        Miller, C. A., Tan, X., Wilson, M., Bhattacharyya, S., & Ludwig, S. (2010). 
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        Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. <i>Plasmid</i>, <i>63</i>(2), 73–78. https://doi.org/10.1016/j.plasmid.2009.11.003
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Revision as of 08:33, 30 September 2024


ERE5


ERE5 is the five tandem repeats of the ERE minimal:BBa_K5107001 to increase the interaction surface of the hormone receptor with the DNA.


Usage and Biology

Previously, in-cell systems have successfully used five tandem repeats of the REs for different human hormonal receptors[1]. Inserted in plasmids such as those we used for our in cell-assay, it is shown efficient to express Lac-Z when the associated hormone-receptor complex is present in the cell. In this manner, hormone work as trancription activator.


Figure 1:Schematic overview of the in cell system which uses the Lac-Z as reporter gene when hormone/edc i spresent in the cell.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 28
    Illegal PstI site found at 45
    Illegal PstI site found at 85
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 28
    Illegal PstI site found at 45
    Illegal PstI site found at 85
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 28
    Illegal PstI site found at 45
    Illegal PstI site found at 85
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 28
    Illegal PstI site found at 45
    Illegal PstI site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]



References

  1. Miller, C. A., Tan, X., Wilson, M., Bhattacharyya, S., & Ludwig, S. (2010). Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Plasmid, 63(2), 73–78. https://doi.org/10.1016/j.plasmid.2009.11.003