Difference between revisions of "Part:BBa K5415002:Design"

 
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<h5>Two sets of triple HRE containing oligonucleotide from EP0 promoter</h5>
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<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
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<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
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<p></p>
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<h5>Post-EcoRV CUT</h5>
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<p>gat</p>
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<p></p>
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<h5>CYC1 promoter from pRL_yEGFP_hc</h5>
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<p>gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat</p>
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<p></p>
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<h5>yeBFP2</h5>
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<p>ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGTGA</p>
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<p>AGGTGATGCAACAAATGGTAA<p>ATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGGG</p>
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<p>TTCAGTGCTTTGCCAGATATCCAGATCACATGAAACAACATGATT<p>TCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAA</p>
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<p>GATGACGGTACATACAAAACAAGAGCTGAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAA<p>TTGAAGGGCGTTGATTTTAAGGAAGATGGTAA</p>
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<p>CATCCTCGGTCATAAGCTAGAATACAATTTCAATTCTCATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAA<p>AATTAGAC</p>
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<p>ATAATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT</p>
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<p>CAATCTGTTTTAT<p>CAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTGGAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATATAAGTAG</p>
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<p></p>
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<h5>ADH2 terminator</h5>
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<p>gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag</p>
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<p>ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc</p>
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<p>agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat</p>

Revision as of 08:28, 30 September 2024

Two sets of triple HRE containing oligonucleotide from EP0 promoter

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

Post-EcoRV CUT

gat

CYC1 promoter from pRL_yEGFP_hc

gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat

yeBFP2

ATGGTGTCAAAAGGTGAAGAATTGTTCACAGGTGTTGTTCCAATTCTAGTTGAATTAGATGGTGACGTGAATGGTCATAAGTTTTCAGTTAGAGGTGAAGGTGA

AGGTGATGCAACAAATGGTAA<p>ATTGACATTGAAGTTTATTTGTACAACCGGTAAGTTGCCAGTTCCGTGGCCAACTCTAGTTACTACATTGTCTCATGGGG

TTCAGTGCTTTGCCAGATATCCAGATCACATGAAACAACATGATT<p>TCTTCAAATCTGCTATGCCAGAAGGTTATGTTCAAGAAAGAACCATTTTTTTTAAA

GATGACGGTACATACAAAACAAGAGCTGAAGTTAAGTTCGAAGGTGATACTTTGGTTAATCGCATAGAA<p>TTGAAGGGCGTTGATTTTAAGGAAGATGGTAA

CATCCTCGGTCATAAGCTAGAATACAATTTCAATTCTCATAATATCTATATTATGGCTGTTAAACAAAAGAATGGTATTAAGGTTAATTTCAA<p>AATTAGAC

ATAATGTCGAAGATGGTTCAGTCCAATTAGCTGATCACTATCAACAAAATACCCCTATTGGTGATGGTCCTGTTTTATTGCCCGATTCTCATTATTTGTCAACT

CAATCTGTTTTAT<p>CAAAAGATCCAAACGAAAAACGTGATCATATGGTTTTGTTGGAgTTCAGAACTGCCGCAGGAATTACTTTAGGTATGGATGAACTATATAAGTAG

ADH2 terminator

gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaaag

ataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtagcttc

agcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat