Difference between revisions of "Part:BBa K5415001:Design"

(sequence data)
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<h5>Triple HRE containing oligonucleotide from EP0 promoter:</h5>
 
<h5>Triple HRE containing oligonucleotide from EP0 promoter:</h5>
 
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
 
<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
 +
<p></p>
 
<p></p>
 
<p></p>
 
<h5>Post-EcoPV cut:</h5>
 
<h5>Post-EcoPV cut:</h5>
 
<p>gat</p>
 
<p>gat</p>
 +
<p></p>
 
<p></p>
 
<p></p>
 
<h5>CYC1 promoter from pRL_yEGFP_hc:</h5>
 
<h5>CYC1 promoter from pRL_yEGFP_hc:</h5>
 
<p>gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat</p>
 
<p>gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat</p>
 +
<p></p>
 
<p></p>
 
<p></p>
 
<h5>yeGFP:</h5>
 
<h5>yeGFP:</h5>
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<p>agatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgcc</p>
 
<p>agatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgcc</p>
 
<p>ttatccaaagatccaaacgaaaagagagatcacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaattgtacaaataa</p>
 
<p>ttatccaaagatccaaacgaaaagagagatcacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaattgtacaaataa</p>
 +
<p></p>
 
<p></p>
 
<p></p>
 
<h5>ADH2 terminator:</h5>
 
<h5>ADH2 terminator:</h5>

Revision as of 08:17, 30 September 2024

Triple HRE containing oligonucleotide from EP0 promoter:

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

Post-EcoPV cut:

gat

CYC1 promoter from pRL_yEGFP_hc:

gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat

yeGFP:

atgtctaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaag

gtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttca

atgttttgcgagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgac

ggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttag

gtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattga

agatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgcc

ttatccaaagatccaaacgaaaagagagatcacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaattgtacaaataa

ADH2 terminator:

gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaa

agataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtag

cttcagcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat