Difference between revisions of "Part:BBa K5415001:Design"

 
(sequence data)
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<h5>Triple HRE containing oligonucleotide from EP0 promoter:</h5>
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<p>TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC</p>
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<p></p>
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<h5>Post-EcoPV cut:</h5>
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<p>gat</p>
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<p></p>
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<h5>CYC1 promoter from pRL_yEGFP_hc:</h5>
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<p>gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat</p>
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<p></p>
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<h5>yeGFP:</h5>
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<p>atgtctaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaag</p>
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<p>gtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttca</p>
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<p>atgttttgcgagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgac</p>
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<p>ggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttag</p>
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<p>gtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattga</p>
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<p>agatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgcc</p>
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<p>ttatccaaagatccaaacgaaaagagagatcacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaattgtacaaataa</p>
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<p></p>
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<h5>ADH2 terminator:</h5>
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<p>gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaa</p>
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<p>agataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtag</p>
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<p>cttcagcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat</p>

Revision as of 08:14, 30 September 2024

Triple HRE containing oligonucleotide from EP0 promoter:

TCGACAAGCTTGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAGCCCTACGTGCTGTCTCAC

Post-EcoPV cut:

gat

CYC1 promoter from pRL_yEGFP_hc:

gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat

yeGFP:

atgtctaaaggtgaagaattattcactggtgttgtcccaattttggttgaattagatggtgatgttaatggtcacaaattttctgtctccggtgaaggtgaag

gtgatgctacttacggtaaattgaccttaaaatttatttgtactactggtaaattgccagttccatggccaaccttagtcactactttcggttatggtgttca

atgttttgcgagatacccagatcatatgaaacaacatgactttttcaagtctgccatgccagaaggttatgttcaagaaagaactatttttttcaaagatgac

ggtaactacaagaccagagctgaagtcaagtttgaaggtgataccttagttaatagaatcgaattaaaaggtattgattttaaagaagatggtaacattttag

gtcacaaattggaatacaactataactctcacaatgtttacatcatggctgacaaacaaaagaatggtatcaaagttaacttcaaaattagacacaacattga

agatggttctgttcaattagctgaccattatcaacaaaatactccaattggtgatggtccagtcttgttaccagacaaccattacttatccactcaatctgcc

ttatccaaagatccaaacgaaaagagagatcacatggtcttgttagaatttgttactgctgctggtattacccatggtatggatgaattgtacaaataa

ADH2 terminator:

gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaataaa

agataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatggtag

cttcagcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat