Difference between revisions of "Part:BBa K5036040"

Revision as of 08:04, 30 September 2024

CRISPR multiplexing system (Nanog gRNA-HHR-gRNA operator)

Part Description

CRISPR multiplexing system is a versatile genetic engineering tool, that empowers scientists to simultaneously target and modify multiple genes within an organism. By designing multiple guide RNAs that precisely bind to specific DNA sequences of interest, researchers can efficiently and accurately introduce desired genetic changes. This capability enhances efficiency and precision, making CRISPR a valuable asset for a wide range of biological applications.

Usage

Our CRISPR multiplexing system consists of three components: a guide RNA targeting the Nanog gene to activate endogenous YAP-1 production by (our dCAS9 transcription activator) complex, a guide RNA targeting a sequence to activate the production of mRNA containing the TID switch by (our dCAS9 transcription activator) complex, and the HHR enzyme which mediates self-cleavage to separate these guide RNAs into individual functional units.

this figure illustrates the process our CRISPER multiplexing system which Allow us to fully utilize the capabilities of our dCas9(N/C) Syn-RTK system. .

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 316
1000
COMPATIBLE WITH RFC[1000]

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 ==Usage==
-Our CRISPR multiplexing system consists of three components: a guide RNA targeting the Nanog gene to activate endogenous YAP production by (our dCAS9 transcription activator) complex, a guide RNA targeting a sequence to activate the production of mRNA containing the TID switch by (our dCAS9 transcription activator) complex, and the HHR enzyme which mediates self-cleavage to separate these guide RNAs into individual functional units.
+Our CRISPR multiplexing system consists of three components: a guide RNA targeting the Nanog gene to activate endogenous YAP-1 production by (our dCAS9 transcription activator) complex, a guide RNA targeting a sequence to activate the production of mRNA containing the TID switch by (our dCAS9 transcription activator) complex, and the HHR enzyme which mediates self-cleavage to separate these guide RNAs into individual functional units.
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