Difference between revisions of "Part:BBa K5036037"
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==Usage== | ==Usage== | ||
| − | Our guide RNA is used with NLS to direct dCas9 linked to transcription activators VP64 and GAL4 into the nucleus to target Nanog gene which is located upstream to YAP coding region which induce transcription and increase production of endogenous YAP. | + | Our guide RNA is used with NLS to direct dCas9 linked to transcription activators VP64 and GAL4 into the nucleus to target Nanog gene which is located upstream to YAP-1 coding region which induce transcription and increase production of endogenous YAP-1. |
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Revision as of 08:02, 30 September 2024
U6 promoter- Nanog gRNA3 (scaffold- spacer RNA )
Part Description
This part consists of the U6 promoter, which constantly activates the creation of our Nanog guide RNA, and The Nanog guide RNA comprises a spacer sequence that matches the Nanog DNA sequence, enabling gene editing. Additionally, the guide RNA includes a scaffold sequence that binds to the dCas9 protein, allowing it to interact with the target DNA
Usage
Our guide RNA is used with NLS to direct dCas9 linked to transcription activators VP64 and GAL4 into the nucleus to target Nanog gene which is located upstream to YAP-1 coding region which induce transcription and increase production of endogenous YAP-1.
This figure illustrates the function of Nanog gRNA in our model.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]