Difference between revisions of "Part:BBa K5207004"

 
 
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It belongs to the CYP76AH subfamily of cytochrome P450 oxygenases. It is a hybrid catalytic P450 that catalyzes the hydroxylation of ferredoxin at the C11 position and the carbonylation of tanshinone at the C7 position, generating 11-hydroxyferredoxin, lupulin, and 11-hydroxylupulin.The carbonylation function at the C7 position allows for the extension of the tanshinone biosynthesis pathway into an additional branched chain, resulting in the formation of a complex catalytic network. We inserted it into pYTK001 by method above.
 
It belongs to the CYP76AH subfamily of cytochrome P450 oxygenases. It is a hybrid catalytic P450 that catalyzes the hydroxylation of ferredoxin at the C11 position and the carbonylation of tanshinone at the C7 position, generating 11-hydroxyferredoxin, lupulin, and 11-hydroxylupulin.The carbonylation function at the C7 position allows for the extension of the tanshinone biosynthesis pathway into an additional branched chain, resulting in the formation of a complex catalytic network. We inserted it into pYTK001 by method above.
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5207004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5207004 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K5207004 parameters</partinfo>
 
<partinfo>BBa_K5207004 parameters</partinfo>
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===Usage and Biology===
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<html>
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<body>
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<figure>
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<div class = "center">
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<center><img src = "https://static.igem.wiki/teams/5207/parts/1.jpg" style = "width:600px"></center>
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</div>
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<figcaption><center>Figure 1. Validation plot of target gene gel electrophoresis</center></figcaption>
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</figure>
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</body>
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</html>
 
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Latest revision as of 07:49, 30 September 2024


CYP76AH3

It belongs to the CYP76AH subfamily of cytochrome P450 oxygenases. It is a hybrid catalytic P450 that catalyzes the hydroxylation of ferredoxin at the C11 position and the carbonylation of tanshinone at the C7 position, generating 11-hydroxyferredoxin, lupulin, and 11-hydroxylupulin.The carbonylation function at the C7 position allows for the extension of the tanshinone biosynthesis pathway into an additional branched chain, resulting in the formation of a complex catalytic network. We inserted it into pYTK001 by method above.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 695
    Illegal SapI.rc site found at 1445


Usage and Biology

Figure 1. Validation plot of target gene gel electrophoresis