Difference between revisions of "Part:BBa K5207003"
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It is from the CYP76AH subfamily of cytochrome P450 oxygenases. It is a ferredoxin synthase that catalyzes the C12 hydroxylation of miltiradiene. We inserted it into pYTK001 by method above. | It is from the CYP76AH subfamily of cytochrome P450 oxygenases. It is a ferredoxin synthase that catalyzes the C12 hydroxylation of miltiradiene. We inserted it into pYTK001 by method above. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K5207003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5207003 SequenceAndFeatures</partinfo> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K5207003 parameters</partinfo> | <partinfo>BBa_K5207003 parameters</partinfo> | ||
| + | <!-- --> | ||
| + | |||
| + | <!-- Add more about the biology of this part here--> | ||
| + | ===Usage and Biology=== | ||
| + | <html> | ||
| + | <body> | ||
| + | <figure> | ||
| + | <div class = "center"> | ||
| + | <center><img src = "https://static.igem.wiki/teams/5207/parts/1.jpg" style = "width:600px"></center> | ||
| + | </div> | ||
| + | <figcaption><center>Figure 1. Validation plot of target gene gel electrophoresis</center></figcaption> | ||
| + | </figure> | ||
| + | </body> | ||
| + | </html> | ||
<!-- --> | <!-- --> | ||
Latest revision as of 07:46, 30 September 2024
CYP76AH1
It is from the CYP76AH subfamily of cytochrome P450 oxygenases. It is a ferredoxin synthase that catalyzes the C12 hydroxylation of miltiradiene. We inserted it into pYTK001 by method above.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 18
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1417
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
