Difference between revisions of "Part:BBa K5195010"
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Base pairs: 1425 | Base pairs: 1425 | ||
Usage and Biology: | Usage and Biology: | ||
In order to be able to quantify how much DUX4-DBD protein would be produced in cells upon transfection, we constructed a coding sequence consisting of an mNeonGreen (mNG) sequence followed by a Peptide 2A (P2A) linker upstream of the DUX4-DBD. The P2A linker causes ribosomal “skipping” during translation, and this effectively separates the mNG and DUX4-DBD proteins. In designing the coding sequence in this way, we enabled quantification of DUX4-DBD without directly attaching another large protein to it. | In order to be able to quantify how much DUX4-DBD protein would be produced in cells upon transfection, we constructed a coding sequence consisting of an mNeonGreen (mNG) sequence followed by a Peptide 2A (P2A) linker upstream of the DUX4-DBD. The P2A linker causes ribosomal “skipping” during translation, and this effectively separates the mNG and DUX4-DBD proteins. In designing the coding sequence in this way, we enabled quantification of DUX4-DBD without directly attaching another large protein to it. | ||
Revision as of 07:46, 30 September 2024
Description: Base pairs: 1425
Usage and Biology: In order to be able to quantify how much DUX4-DBD protein would be produced in cells upon transfection, we constructed a coding sequence consisting of an mNeonGreen (mNG) sequence followed by a Peptide 2A (P2A) linker upstream of the DUX4-DBD. The P2A linker causes ribosomal “skipping” during translation, and this effectively separates the mNG and DUX4-DBD proteins. In designing the coding sequence in this way, we enabled quantification of DUX4-DBD without directly attaching another large protein to it.