Difference between revisions of "Part:BBa K5207000"

 
 
Line 5: Line 5:
 
This gene is responsible for the control of geranylgeranyl diphosphate synthase synthesis in the downstream geranylgeranyl MEP pathway of Salvia miltiorrhiza[3]. We designed primer F: GCATCGTCTCATCGGTCTCAT(ATG) + CDS-5' end and primer R: ATGCCGTCTCAGGTCTCAGGAT + CDS-3' end of the junction sequence containing BsmBI enzyme site, and cloned the sequences containing the SmGGPPS of the target gene by PCR reaction. The sequence containing the target gene SmGGPPS was cloned by PCR and inserted into vector pYTK001.
 
This gene is responsible for the control of geranylgeranyl diphosphate synthase synthesis in the downstream geranylgeranyl MEP pathway of Salvia miltiorrhiza[3]. We designed primer F: GCATCGTCTCATCGGTCTCAT(ATG) + CDS-5' end and primer R: ATGCCGTCTCAGGTCTCAGGAT + CDS-3' end of the junction sequence containing BsmBI enzyme site, and cloned the sequences containing the SmGGPPS of the target gene by PCR reaction. The sequence containing the target gene SmGGPPS was cloned by PCR and inserted into vector pYTK001.
  
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
−
<!-- -->
 
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5207000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5207000 SequenceAndFeatures</partinfo>
Line 16: Line 13:
 
===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K5207000 parameters</partinfo>
 
<partinfo>BBa_K5207000 parameters</partinfo>
 +
<!-- -->
 +
 +
<!-- Add more about the biology of this part here-->
 +
===Usage and Biology===
 +
<html>
 +
<body>
 +
<figure>
 +
<div class = "center">
 +
<center><img src = "https://static.igem.wiki/teams/5207/parts/1.jpg" style = "width:600px"></center>
 +
</div>
 +
<figcaption><center>Figure 1. Validation plot of target gene gel electrophoresis</center></figcaption>
 +
</figure>
 +
</body>
 +
</html>
 
<!-- -->
 
<!-- -->

Latest revision as of 07:43, 30 September 2024


SmGGPPS

This gene is responsible for the control of geranylgeranyl diphosphate synthase synthesis in the downstream geranylgeranyl MEP pathway of Salvia miltiorrhiza[3]. We designed primer F: GCATCGTCTCATCGGTCTCAT(ATG) + CDS-5' end and primer R: ATGCCGTCTCAGGTCTCAGGAT + CDS-3' end of the junction sequence containing BsmBI enzyme site, and cloned the sequences containing the SmGGPPS of the target gene by PCR reaction. The sequence containing the target gene SmGGPPS was cloned by PCR and inserted into vector pYTK001.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 166
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Figure 1. Validation plot of target gene gel electrophoresis