Difference between revisions of "Part:BBa K5226052"

 
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<partinfo>BBa_K5226052 short</partinfo>
 
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===Sequence and Features===
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<partinfo>BBa_K5226052 SequenceAndFeatures</partinfo>
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<br>
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===Introduction===
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One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to obtain ,<i>Halomonas</i> strains that can produce tyrian purple. We chose to introduce four enzymes that is either necessary or beneficial to the production of tyrian purple.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This part is a <b>native ribosomal binding site of stth</b>, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts to facilitate the expression of Stth.
  
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<span class='h3bb'>Sequence and Features</span>
 
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<partinfo>BBa_K5226052 SequenceAndFeatures</partinfo>
 
  
  

Revision as of 07:13, 30 September 2024


321(C+cysNC) backbone

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 2528
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NgoMIV site found at 2981
    Illegal NgoMIV site found at 3866
    Illegal NgoMIV site found at 4977
    Illegal NgoMIV site found at 5101
    Illegal AgeI site found at 2037
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 2103
    Illegal SapI.rc site found at 2171


Introduction

One of the goals of iGEM SCUT-China-A is to use synthetic biology tools to obtain ,Halomonas strains that can produce tyrian purple. We chose to introduce four enzymes that is either necessary or beneficial to the production of tyrian purple.

Usage and Biology

This part is a native ribosomal binding site of stth, which has not yet been characterized. We assessed the ease of plasmid construction and ultimately chose to utilize this part in the composite parts to facilitate the expression of Stth.