Difference between revisions of "Part:BBa K5034201"
Line 4: | Line 4: | ||
__TOC__ | __TOC__ | ||
+ | ===Description=== | ||
+ | |||
+ | The pYYDT plasmid is a synthetic plasmid backbone used in various genetic engineering applications in microbial systems. | ||
===Sequence and Features=== | ===Sequence and Features=== | ||
<partinfo>BBa_K5034201 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5034201 SequenceAndFeatures</partinfo> | ||
− | === | + | ===Applications=== |
− | + | 1. It can be used in microbial fuel cells to study and enhance the extracellular electron transfer capabilities such as in <i>S. oneidensis</i>. | |
+ | 2. It can be used as a backbone for CRISPR-based genome editing systems, enabling precise genetic modifications. | ||
− | |||
===Reference=== | ===Reference=== | ||
Yang, Y., Wu, Y., Hu, Y., Cao, Y., Poh, C. L., Cao, B., & Song, H. (2015). Engineering Electrode-Attached Microbial Consortia for High-Performance Xylose-Fed Microbial Fuel Cell. ACS Catalysis, 5(11), 6937-6945. DOI: 10.1021/acscatal.5b01733. | Yang, Y., Wu, Y., Hu, Y., Cao, Y., Poh, C. L., Cao, B., & Song, H. (2015). Engineering Electrode-Attached Microbial Consortia for High-Performance Xylose-Fed Microbial Fuel Cell. ACS Catalysis, 5(11), 6937-6945. DOI: 10.1021/acscatal.5b01733. |
Revision as of 06:50, 30 September 2024
pBBR1MCS-terminator plasmid
Description
The pYYDT plasmid is a synthetic plasmid backbone used in various genetic engineering applications in microbial systems.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4981
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2834
Illegal NotI site found at 4987 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 4981
Illegal BglII site found at 3580 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 4981
Illegal suffix found at 2 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 4981
Plasmid lacks a suffix.
Illegal XbaI site found at 4996
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NgoMIV site found at 562
Illegal NgoMIV site found at 4244
Illegal NgoMIV site found at 4527
Illegal AgeI site found at 402 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Applications
1. It can be used in microbial fuel cells to study and enhance the extracellular electron transfer capabilities such as in S. oneidensis. 2. It can be used as a backbone for CRISPR-based genome editing systems, enabling precise genetic modifications.
Reference
Yang, Y., Wu, Y., Hu, Y., Cao, Y., Poh, C. L., Cao, B., & Song, H. (2015). Engineering Electrode-Attached Microbial Consortia for High-Performance Xylose-Fed Microbial Fuel Cell. ACS Catalysis, 5(11), 6937-6945. DOI: 10.1021/acscatal.5b01733.