Difference between revisions of "Part:BBa K5034201:Design"
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
From <i>Shewanella oneidensis</i>
+
From <i>Shewanella oneidensis</i>.
  
 
===References===
 
===References===
  
 
Yang, Y., Wu, Y., Hu, Y., Cao, Y., Poh, C. L., Cao, B., & Song, H. (2015). Engineering Electrode-Attached Microbial Consortia for High-Performance Xylose-Fed Microbial Fuel Cell. ACS Catalysis, 5(11), 6937-6945. https://doi.org/10.1021/acscatal.5b01733
 
Yang, Y., Wu, Y., Hu, Y., Cao, Y., Poh, C. L., Cao, B., & Song, H. (2015). Engineering Electrode-Attached Microbial Consortia for High-Performance Xylose-Fed Microbial Fuel Cell. ACS Catalysis, 5(11), 6937-6945. https://doi.org/10.1021/acscatal.5b01733

Latest revision as of 06:46, 30 September 2024


pBBR1MCS-terminator plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4981
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2834
    Illegal NotI site found at 4987
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 4981
    Illegal BglII site found at 3580
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 4981
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 4981
    Plasmid lacks a suffix.
    Illegal XbaI site found at 4996
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 4244
    Illegal NgoMIV site found at 4527
    Illegal AgeI site found at 402
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

We synthesized the fragment using a chemical synthesis method.


Source

From Shewanella oneidensis.

References

Yang, Y., Wu, Y., Hu, Y., Cao, Y., Poh, C. L., Cao, B., & Song, H. (2015). Engineering Electrode-Attached Microbial Consortia for High-Performance Xylose-Fed Microbial Fuel Cell. ACS Catalysis, 5(11), 6937-6945. https://doi.org/10.1021/acscatal.5b01733