Difference between revisions of "Part:BBa K5071004"

 
 
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BGCI-5
 
  
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===Usage and Biology===
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===Functional Parameters===
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    <h2>Composite part BBa_K5071004 (BGCI-5)</h2>
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    <h3>Name: BGCI-5</h3>
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    <p><strong>Base Pairs:</strong> 800 bp</p>
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    <p><strong>Origin:</strong> Bacteriovoracaceae</p>
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    <h3>Usage and Biology</h3>
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        BGCI-5 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.
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    <h3>Cultivation</h3>
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        We used PCR to amplify the BGCI-5 gene, with a length of 800 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pETDuet-BGCI-gene456.
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        <img src="https://static.igem.wiki/teams/5071/bba-k5071004/1.jpg" alt="Fig 1. The purpose segment of plasmid pRSFuet-BGC1-gene456">
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        <div class="caption">Fig 1. The purpose segment of plasmid pRSFuet-BGC1-gene456</div>
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Latest revision as of 04:40, 30 September 2024

_ BGCI-5



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 640
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 640
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 399
    Illegal BamHI site found at 225
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 640
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 640
  • 1000
    COMPATIBLE WITH RFC[1000]


BBa_K5071004 (BGCI-5)

Composite part BBa_K5071004 (BGCI-5)

Name: BGCI-5

Base Pairs: 800 bp

Origin: Bacteriovoracaceae

Usage and Biology

BGCI-5 is a gene identified within a predicted terpene biosynthesis gene cluster, with bioinformatic tools suggesting a potential role in terpene production. Although its precise function remains unknown, sequence analysis indicates the presence of conserved domains common to enzymes involved in secondary metabolite pathways, highlighting its potential importance in the biosynthesis of terpenoids. Further experimental validation is required to elucidate its specific role.

Cultivation

We used PCR to amplify the BGCI-5 gene, with a length of 800 bp. Fig 1 shows a band consistent with the target size, indicating that the target gene was successfully amplified. After agarose gel electrophoresis and gel recovery, homologous recombination was used to obtain the recombinant plasmid pETDuet-BGCI-gene456.

Fig 1. The purpose segment of plasmid pRSFuet-BGC1-gene456
Fig 1. The purpose segment of plasmid pRSFuet-BGC1-gene456