Difference between revisions of "Part:BBa K5291039:Design"

Latest revision as of 04:29, 30 September 2024

P_QJ: cumate-inducible promoter

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Leveraging the P_QJ promoter's responsiveness to cumate, coupled with cumate's established environmental stability and non-toxic profile, we can strategically deploy the P_QJ promoter to activate the suicide system in engineered bacteria, achieving precise control over bacterial self-termination.

Source

This Promoter was derived from the literature: A Synthetic Cumate-Inducible Promoter for Graded and Homogenous Gene Expression in Pseudomonas aeruginosa

References

Klotz A, Kaczmarczyk A, Jenal U. A Synthetic Cumate-Inducible Promoter for Graded and Homogenous Gene Expression in Pseudomonas aeruginosa. Appl Environ Microbiol. 2023 Jun 28;89(6):e0021123. doi: 10.1128/aem.00211-23. Epub 2023 May 18. PMID: 37199671; PMCID: PMC10304978.

@@ Line 7: / Line 7: @@
 ===Design Notes===
-Given the stability and non-toxic nature of cumate in the environment, cumate can be selected as an inducer to initiate the self-destruction pathway in engineered bacteria, thereby enabling controlled self-termination.
+Leveraging the P<sub>QJ</sub> promoter's responsiveness to cumate, coupled with cumate's established environmental stability and non-toxic profile, we can strategically deploy the P<sub>QJ</sub> promoter to activate the suicide system in engineered bacteria, achieving precise control over bacterial self-termination.
@@ Line 16: / Line 16: @@
 ===References===
+Klotz A, Kaczmarczyk A, Jenal U. A Synthetic Cumate-Inducible Promoter for Graded and Homogenous Gene Expression in Pseudomonas aeruginosa. Appl Environ Microbiol. 2023 Jun 28;89(6):e0021123. doi: 10.1128/aem.00211-23. Epub 2023 May 18. PMID: 37199671; PMCID: PMC10304978.