Difference between revisions of "Part:BBa K174017"

Revision as of 22:58, 21 October 2009

CadA promoter with CzrA binding site

In B. subtilis CadA system works as an efflux system to take the metals out in the cell. It is normally repressed by the CzrA (formely YozA) repressor protein.

When metals such as copper, zinc and arsenic are present inside the cell, they can bind to CzrA and derepress the promoter expressing CadA. Hence metals are taken out by the efflux system.

By utilising the promoter from the CadA system, we created a metal sensor, that responds to at least these three metals.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 166
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K174017 short</partinfo>
-In ''B. subtilis'' CadA system works as an efflux system to take the metals out in the cell. It is normally repressed by CzrA repressor protein. When certain metals get inside the cell, they can bind to CzrA and derepress the promoter expressing CadA. Hence metals are taken out by the efflux system.
+In ''B. subtilis'' CadA system works as an efflux system to take the metals out in the cell. It is normally repressed by the CzrA (formely YozA) repressor protein.
+When metals such as copper, zinc and arsenic are present inside the cell, they can bind to CzrA and derepress the promoter expressing CadA. Hence metals are taken out by the efflux system.
+By utilising the promoter from the CadA system, we created a metal sensor, that responds to at least these three metals.
-By taking the promoter of CadA system, we created a generic metal sensor. Hence when it is combined with coding sequences of other genes, it will regulate the expression of these genes upon sensing metals.
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