Difference between revisions of "Part:BBa K5520004"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | AiiA degrades AHLs, a class of signaling molecules that induce quorum sensing. Quorum sensing triggers the expression of virulence factors in pathogens, including Aeromonas hydrophila, an aquatic bacterium that threatens aquaculture (Coquant et al., 2020; Hlordzi et al., 2020). High expression of AiiA in the probiotic B. subtilis reduces the virulence of A. hydrophila through quorum quenching and saves aquatic animals. | ||
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| + | <center><img src = "https://static.igem.wiki/teams/5208/parts/00-2.png" style = "width:300px"></center> | ||
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| + | <figcaption><center>Figure 2. Gel electrophoresis of PCR product of AiiA. </center></figcaption> | ||
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Revision as of 03:44, 30 September 2024
pT7-LacO-His-CDA
This part contains BBa_K2406020, BBa_B0034, BBa_K5520002 and BBa_K731721. BBa_B0034 contains a natural ribosome binding site (RBS) to facilitate the expression of downstream genes. By inducing efficient expression of the CDA protein through the addition of IPTG, the protein can subsequently be purified using the His tag for further enzyme activity tests and product polymerization.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 149
Illegal NheI site found at 897 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 182
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 641