Difference between revisions of "Part:BBa K5520003:Design"

 
 
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<partinfo>BBa_K5520003 short</partinfo>
  
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<partinfo>BBa_K5520003 SequenceAndFeatures</partinfo>
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===Design Notes===
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In the context of conducting an experiment to express and purify CsnB proteins using the pET-28a expression system, the decision to incorporate a 6×His tag was made with the purpose of simplifying the purification process and enabling protein characterization.
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===Source===
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Escherichia coli and Bacillus pumilus
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===References===

Latest revision as of 03:36, 30 September 2024

6×His-CsnB



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 709
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 58
    Illegal AgeI site found at 541
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

In the context of conducting an experiment to express and purify CsnB proteins using the pET-28a expression system, the decision to incorporate a 6×His tag was made with the purpose of simplifying the purification process and enabling protein characterization.



Source

Escherichia coli and Bacillus pumilus


References