Difference between revisions of "Part:BBa K5335025"
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| − | + | To enhance plant immunity, a novel fusion protein, SpyCatcher-VDAL-CPPs, was designed. This construct incorporates a SpyCatcher tag<sub>【1】</sub> for conjugation, VDAL for immune activation<sub>【2】</sub>, and the R9 cell-penetrating peptide for intracellular delivery<sub>【3】</sub>. Functional studies demonstrated the capacity of this fusion protein to penetrate plant cell membranes and induce an ETI response. Molecular characterization and DAB staining confirmed the successful construction and immunogenic activity of the fusion protein. | |
The design was verified as shown in Figure 1. | The design was verified as shown in Figure 1. | ||
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Revision as of 02:33, 30 September 2024
SpyCatcher-VDAL-CPPs (R9-Tag)
To enhance plant immunity, a novel fusion protein, SpyCatcher-VDAL-CPPs, was designed. This construct incorporates a SpyCatcher tag【1】 for conjugation, VDAL for immune activation【2】, and the R9 cell-penetrating peptide for intracellular delivery【3】. Functional studies demonstrated the capacity of this fusion protein to penetrate plant cell membranes and induce an ETI response. Molecular characterization and DAB staining confirmed the successful construction and immunogenic activity of the fusion protein. The design was verified as shown in Figure 1.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]