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SpyCatcher-VDAL-CPPs is a plant immune-activating protein engineered to contain a Spycatcher tag and an R9 cell-penetrating peptide. It can penetrate plant cell membranes and induce an ETI response intracellularly.During the experiment, we successfully constructed the fusion protein and verified its function at the molecular level. Additionally, we confirmed its immunomodulatory activity using DAB-based reactive oxygen species staining.
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    To enhance plant immunity, a novel fusion protein, SpyCatcher-VDAL-CPPs, was designed. This construct incorporates a SpyCatcher tag<sub>【1】</sub> for conjugation, VDAL for immune activation<sub>【2】</sub>, and the R9 cell-penetrating peptide for intracellular delivery<sub>【3】</sub>. Functional studies demonstrated the capacity of this fusion protein to penetrate plant cell membranes and induce an ETI response. Molecular characterization and DAB staining confirmed the successful construction and immunogenic activity of the fusion protein.
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The design was verified as shown in Figure 1.  
 
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<center><img src="https://static.igem.wiki/teams/5335/plant-cpps/4-3.png" style="width:50%; "></center>
 
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<center><b>Figure 1. Experimental circuit design diagram </b> </center>
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Revision as of 02:33, 30 September 2024


SpyCatcher-VDAL-CPPs (R9-Tag) 

    To enhance plant immunity, a novel fusion protein, SpyCatcher-VDAL-CPPs, was designed. This construct incorporates a SpyCatcher tag【1】 for conjugation, VDAL for immune activation【2】, and the R9 cell-penetrating peptide for intracellular delivery【3】. Functional studies demonstrated the capacity of this fusion protein to penetrate plant cell membranes and induce an ETI response. Molecular characterization and DAB staining confirmed the successful construction and immunogenic activity of the fusion protein.

The design was verified as shown in Figure 1. 无标题文档


Figure 1. Experimental circuit design diagram

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]