Difference between revisions of "Part:BBa K5520002:Design"
 
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===Design Notes===
 
===Design Notes===
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We propose that the second step of the reaction catalyzes the generation of chitosan from chitosan to chitooligosaccharides during the generation of chitosan from chitin. We utilize the chitosanase CsnB to degrade chitosan in order to obtain chitooligosaccharides with high added value. , which can somewhat alter the reaction equilibrium of the precursor reaction and thus improve the utilization of the reaction substrate. We cloned the CsnB gene into the pET-28a vector to obtain the PET-28a-CsnB recombinant plasmid, which was transformed into E.coli BL21 (DE3) for overexpression of chitosanase CsnB.
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In designing an experiment to express and purify CDA protein by the PET-28a system, the inclusion of a 6×His tag was intended to facilitate subsequent protein purification and identification.
  
  
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===Source===
 
===Source===
  
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Marine Bacterium Bacilius SP. BY01
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Escherichia coli and Bacillus pumilus
  
 
===References===
 
===References===

Latest revision as of 02:31, 30 September 2024

6*His-CDA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 544


Design Notes

In designing an experiment to express and purify CDA protein by the PET-28a system, the inclusion of a 6×His tag was intended to facilitate subsequent protein purification and identification.


Source

Escherichia coli and Bacillus pumilus

References