Difference between revisions of "Part:BBa K5324015:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | This sequence was codon optimized for expression in E.coli. Restriction cut sites were avoided for BsaI, BsmI, and SapI. | |
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | + | Destructin-1 originated from P. destructans. | |
===References=== | ===References=== | ||
| + | 1 - Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112 <br> | ||
| + | 2 - U.S. National Library of Medicine. (n.d.). RecName: Full=subtilisin-like protease 2; AltName: Full=destructin-1;. National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/protein/L8FSM5.1 | ||
Latest revision as of 02:23, 30 September 2024
Destructin-1 Expression Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 102 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 879
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was codon optimized for expression in E.coli. Restriction cut sites were avoided for BsaI, BsmI, and SapI.
Source
Destructin-1 originated from P. destructans.
References
1 - Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112
2 - U.S. National Library of Medicine. (n.d.). RecName: Full=subtilisin-like protease 2; AltName: Full=destructin-1;. National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/protein/L8FSM5.1