Difference between revisions of "Part:BBa K5324012:Design"
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===References===
 
===References===
 
1 - Spink8 serine peptidase inhibitor Kazal Type 8 (putative) [homo sapiens (human)] - gene - NCBI National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=646424.
 
1 - Spink8 serine peptidase inhibitor Kazal Type 8 (putative) [homo sapiens (human)] - gene - NCBI National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=646424.
 +
2 - Thomas, S., Holland, I.B. and Schmitt, L. (2014) ‘The type 1 secretion pathway — the hemolysin system and beyond’, Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1843(8), pp. 1629–1641. doi:10.1016/j.bbamcr.2013.09.017.

Revision as of 02:02, 30 September 2024


SPINK8 Secretion Device


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

SPINK8 is codon optimized for E. coli. Bsal and Bsml restriction cut site were removed with mutations.


Source

SPINK8 originated from Homo sapiens.

References

1 - Spink8 serine peptidase inhibitor Kazal Type 8 (putative) [homo sapiens (human)] - gene - NCBI National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/gene?Db=gene&Cmd=DetailsSearch&Term=646424. 2 - Thomas, S., Holland, I.B. and Schmitt, L. (2014) ‘The type 1 secretion pathway — the hemolysin system and beyond’, Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1843(8), pp. 1629–1641. doi:10.1016/j.bbamcr.2013.09.017.