Difference between revisions of "Part:BBa K196004"

Latest revision as of 22:52, 21 October 2009

HfsG + HfsH proteins from Caulobacter crescentus

Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically. Besides the bacterium is able to synthesize a strong glue. This glue is mainly composed of polysaccharide. Different proteins are required in the holdfast synthesis, export and attachment in C.crescentus (for more details, please refer to the "introduction" section). In our project, we wanted to implement the glue production in Escherichia coli. As E. coli possesses homolog proteins, we decid to insert the hfsG and hfsH genes in a plasmid. HfsG[1]is a glycosyltransferase and HfsH [2] is a carbohydrate esterase. Here is the biobrick composed og the hfsG and hfsH genes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1470
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 480
Illegal AgeI site found at 379
Illegal AgeI site found at 864
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K196004 short</partinfo>
-Caulobacter crescentus is an aquatic, Gram-negative bacterium that divides asymmetrically and is able to synthetize a strong glue. This glue is mainly made of a polysaccharide. There are different proteins needed to synthetize, export and attach it to the stalk of Caulobacter. To see the hole system, please see this page [http://jb.asm.org/cgi/content/full/190/21/7219/F8]. In our project, we would like this glue to be produced by Escherichia coli. As E. coli does have homolog genes for many of these proteins, but not for HfsG and HfsH, we decided to create a plasmid including only the genes coding for these two proteins. HfsG is a glycosyltransferase and HfsH is a carbohydrate esterase. Here you have a part made of the Biobricks HfsG (BBa_K196002) and HfsH (BBa_K196003).
+<i>Caulobacter crescentus</i> is an aquatic, Gram-negative bacterium that divides asymmetrically. Besides the bacterium is able to synthesize a strong glue. This glue is mainly composed of polysaccharide. Different proteins are required in the holdfast synthesis, export and attachment in <i>C.crescentus</i> (for more details, please refer to the "introduction" section). In our project, we wanted to implement the glue production in <i>Escherichia coli</i>. As <i>E. coli</i> possesses homolog proteins, we decid to insert the hfsG and hfsH genes in a plasmid. HfsG[https://parts.igem.org/Part:BBa_K196002:Design]is a glycosyltransferase and HfsH [https://parts.igem.org/Part:BBa_K196002:Design] is a carbohydrate esterase. Here is the biobrick composed og the hfsG and hfsH genes.
 <!-- Add more about the biology of this part here