Difference between revisions of "Part:BBa K5324012"
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<partinfo>BBa_K5324012 short</partinfo> | <partinfo>BBa_K5324012 short</partinfo> | ||
| − | + | This composite part produces and secretes SPINK6, a serine protease inhibitor. The SPINK6 sequence on its N-terminal has a histidine purification tag connected with a linker. On the C-terminal is the Hly-A secretion signal marking the protein for secretion through the type 1 secretion pathway, which is connected with a TEV signal peptide sequence that is recognized by the TEV protease to be cleaved after secretion. The composite is reinforced with an Anderson promoter, RBS, and terminator. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 00:43, 30 September 2024
SPINK8 Secretion Device
This composite part produces and secretes SPINK6, a serine protease inhibitor. The SPINK6 sequence on its N-terminal has a histidine purification tag connected with a linker. On the C-terminal is the Hly-A secretion signal marking the protein for secretion through the type 1 secretion pathway, which is connected with a TEV signal peptide sequence that is recognized by the TEV protease to be cleaved after secretion. The composite is reinforced with an Anderson promoter, RBS, and terminator. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]