Difference between revisions of "Part:BBa K5324011"
 
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<partinfo>BBa_K5324011 short</partinfo>
 
<partinfo>BBa_K5324011 short</partinfo>
  
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This part is a Kunitz-type serine protease inhibitor that is used for the inhibition of destructan-1 in the circuit. It is medically used for perioperative blood loss and in blood transfusions for patients undergoing cardiopulmonary bypass.
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This composite part produces and secretes aprotinin, a serine protease inhibitor. The aprotinin sequence on its N-terminal has a histidine purification tag connected with a linker. On the C-terminal is the Hly-A secretion signal marking the protein for secretion through the type 1 secretion pathway, which is connected with a TEV signal peptide sequence that is recognized by the TEV protease to be cleaved after secretion. The composite is reinforced with an Anderson promoter, RBS, and terminator.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 00:42, 30 September 2024


Aprotinin Secretion Device

This composite part produces and secretes aprotinin, a serine protease inhibitor. The aprotinin sequence on its N-terminal has a histidine purification tag connected with a linker. On the C-terminal is the Hly-A secretion signal marking the protein for secretion through the type 1 secretion pathway, which is connected with a TEV signal peptide sequence that is recognized by the TEV protease to be cleaved after secretion. The composite is reinforced with an Anderson promoter, RBS, and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]