Difference between revisions of "Part:BBa K5324002:Design"
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===References=== | ===References=== | ||
| − | Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112 | + | 1 - Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112 <br> |
| − | RecName: Full=subtilisin-like protease 1; AltName: Full=destructin-2; National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/protein/939319657. | + | 2 - RecName: Full=subtilisin-like protease 1; AltName: Full=destructin-2; National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/protein/939319657. |
Latest revision as of 22:08, 29 September 2024
Destructin-2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 589
Illegal NgoMIV site found at 694
Illegal NgoMIV site found at 832
Illegal NgoMIV site found at 1054
Illegal AgeI site found at 283 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was codon optimized for expression in E.coli. Restriction cut sites were avoided for BsaI, BsmI, and SapI.
Source
Genomic sequence obtained from GeneBank. Originated from P. destructans.
References
1 - Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112
2 - RecName: Full=subtilisin-like protease 1; AltName: Full=destructin-2; National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/protein/939319657.