Difference between revisions of "Part:BBa K5324003:Design"

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===References===
 
===References===
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Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112  
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1 - Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112 <br>
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RecName: Full=subtilisin-like protease 3; AltName: Full=destructin-3; National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/protein/939319659.
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2 - RecName: Full=subtilisin-like protease 3; AltName: Full=destructin-3; National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/protein/939319659.

Latest revision as of 22:08, 29 September 2024


Destructin-3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 85
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 475
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was codon optimized for expression in E.coli. Restriction cut sites were avoided for BsaI, BsmI, and SapI.


Source

Genomic sequence obtained from GeneBank. Originated from P. destructans.

References

1 - Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112
2 - RecName: Full=subtilisin-like protease 3; AltName: Full=destructin-3; National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/protein/939319659.