Difference between revisions of "Part:BBa K5324003:Design"

 
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===References===
 
===References===
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Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112
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RecName: Full=subtilisin-like protease 3; AltName: Full=destructin-3; National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/protein/939319659.

Revision as of 21:55, 29 September 2024


Destructin-3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 85
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 475
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence was codon optimized for expression in E.coli. Restriction cut sites were avoided for BsaI, BsmI, and SapI.


Source

Genomic sequence obtained from GeneBank. Originated from P. destructans.

References

Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112 RecName: Full=subtilisin-like protease 3; AltName: Full=destructin-3; National Center for Biotechnology Information. Available at: https://www.ncbi.nlm.nih.gov/protein/939319659.