Difference between revisions of "Part:BBa K5415004:Design"

(Source)
(Design Notes)
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<partinfo>BBa_K5415004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5415004 SequenceAndFeatures</partinfo>
  
 
===Design Notes===
 
ccggtagaggtgtggtcaataagagcgacctcatactatacctgagaaagcaacctgacctacaggaaagagttactcaagaataagaattttcgttttaaaacctaagagtcactttaaaatttg
 
tatacacttattttttttataacttatttaataataaaaatcataaatcataagaaattcgcgctctaGTGGTGATGGTGGTGGTGttctgaaaaggggggaaacatagttagatcaggaaattct
 
tcattgttgtagctgttgctctgatctcccagcatggacagcatctcctggaagacctcaggctggccaggttgctgtgctggcggttgttgaacatgttgctcactagaacttgaacgatgatga
 
ggctgctggccctgccactgtggccagacacccacaccctctgctgtccgtgtctggaattgtccggcagtctgtccagtctcaggagcaaagttagatccacgattggtgagactagggtaggca
 
gcagcaccaggcgatgcagttggggcaccagggagggacatggagctgaaggaggatggagtctgaaagctgcccacaccaaactgggaagtacgagtcttagcagtagcctgggtagccacctgc
 
tgggcagaaaagcctgagcgggtagtaggggtccaagttggggttgctccttgggtggggttggagtggcgggaaatctgggccaacatctgtcccgcagaagctgatggctggacaatggttaca
 
ggaggggctaggccactattcctgaaattctctgccggccggggggtaggagggaatgtgttgccctgggagaatagctgttgggtggcagggacagtgctggaggagatgcctttactctgatcc
 
gcattgatgttgtgatagatttctgaaaatcttggatctctatcctgggcaaataaaccatctgacttctcaaggggcttgctgtgttctggtcctgtggttgtcacaggctgaaccacctgggaa
 
tgattgtagctggccagtccatctcttcctggtaccatgtccaattctgtttgctgttgctgctgcctgggtgccagctgtcctgagcccatctccaggggtaaattagctgtgggacctagttgt
 
ggcctctggattgtgttggagagtgtaggccgtggttcttggctagagttcttcacattggtgttggtacagatgatgtactcaatttcatctgagtaagggttctggaaagtaaaggagctggtt
 
ctcatccagagccattcttggttcttagaccggaaccggaacatgacagacagcacttggccttttaatttcactacctgttggaagctgtctcttagaagctgctggtcttcaggatgacaaaat
 
tctacaatattctttcctaagagttcctgtggctggtagccaacagtagccacacagcggtgatccacaaaagtgaagataccctcaatgttgtgtcgggagatgaactctgttggttgacaaaca
 
ttactcatgtctgtacagttgggagaactggttacctgcaatctgccaatggccactaggcaaaacttgcttccctggccagcctctgggtcatcatctgggagggaaacacctgctgggggccag
 
gccctgatgtagcctgtgcagtggaccaccacgaagtgaggttccccatcctttacagagccaagtccattcctgcatctgttcctcacaaagctcagcctattcacagaaactgggtccacagag
 
ctactgccacacctcattcggcaaataaacgatctccttgagcccatacacattctcatggaagactgctgaccttcctttttcactgttccagtctttagatccaggatacgccctgtcagggca
 
ttttctgaagtggaaagctgctcacgaagtttatccacatcatctgggtgcacctgatcatagagtgtgctgccaaaccattcagactgtggctggttcaaaacaggagtcacggagtcagacaca
 
tacaccaccctgcctgtctcacatgagacaataaacagaaagccatctgctgcctccaagatcaaatgtttcagttcctgatcagtgaggaaagacggcttataggagccatcagtggatgtgttg
 
ccagttccccgcaaggacttcatgtgagaaactgccatgcgtaagatggttagcttgtctggttttcgagccagggcactacaggtgggtaccatatctgacagttctgtgatgtaggctgtcatc
 
ttgttccgtcgccgccgttcaatttcactgtgattttccctggcaaaccgctccttatcgttagacatctgatcatcatcacacctcaaaaatttactgttcccttctccatcatcatcaaaatcc
 
agccctggtcgccgcttaatagccctctggacaatggctcctccaccttggattccaggtccagagtttccagaggcaatggctggacccagtgatggtacatctgatgtcatttcggggttggca
 
gtagtcgccgccatcgaattttcaaaaattcttactttttttttggatggacgcaaagaagtttaataatcatattacatgtgtgaaattgttatccgctcacaattccacacaacatatctaatc
 
ttacttatatgttgtggaaatgtaaagagccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccg
 
agcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactag
 
cttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaat
 
taatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgtccctatcagtgatagagatctccctatcagtgatagagatattacttcttattcaaatgtaataaaagt
 
atcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaaccccggatcctattaaaatggagggcgccggcggcgcgaacgacaagaaaaagataagttctgaacgtcgaaa
 
agaaaagtctcgtgatgcagccagatctcggcgaagtaaagaatctgaagttttttatgagcttgctcatcagttgccacttccacataatgtgagttcgcatcttgataaggcctctgtgatgag
 
gcttaccatcagctatttgcgtgtgaggaaacttctggatgctggtgatttggatattgaagatgacatgaaagcacagatgaattgcttttatttgaaagccttggatggttttgttatggttct
 
cacagatgatggtgacatgatttacatttctgataatgtgaacaaatacatgggattaactcagtttgaactaactggacacagtgtgtttgattttactcatccatgtgaccatgaggaaatgag
 
agaaatgcttacacacagaaatggccttgtgaaaaagggtaaagaacaaaacacacagcgaagtttttttctcagaatgaagtgtaccctaactagccgaggaagaactatgaacataaagtctgc
 
aacatggaaggtattgcactgcacaggccacattcacgtatatgataccaacagtaaccaacctcagtgtgggtataagaaaccacctatgacctgcttggtgctgatttgtgaacccattcctca
 
cccatcaaatattgaaattcctttagatagcaagactttcctcagtcgacacagcctggatatgaaattttcttattgtgatgaaagaattaccgaattgatgggatatgagccagaagaactttt
 
aggccgctcaatttatgaatattatcatgctttggactctgatcatctgaccaaaactcatcatgatatgtttactaaaggacaagtcaccacaggacagtacaggatgcttgccaaaagaggtgg
 
atatgtctgggttgaaactcaagcaactgtcatatataacaccaagaactctcaaccacagtgcattgtatgtgtgaattacgttgtgagtggtattattcagcacgacttgattttctcccttca
 
acaaacagaatgtgtccttaaaccggttgaatcttcagatatgaaaatgactcagctattcaccaaagttgaatcagaagatacaagtagcctctttgacaaactcaagaaggaacctgatgcttt
 
aactttgctggccccagccgctggagacacaatcatatctttagattttggcagcaacgacacagaaactgatgaccagcaacttgaggaagtaccattatataatgatgtaatgctcccctcacc
 
caacgaaaaattacagaatataaatttggcaatgtctccattacccaccgctgaaacgccaaagccacttcgaagtagtgctgaccctgcactcaatcaagaagttgcattaaaattagaaccaaa
 
tccagagtcactggaactttcttttaccatgccccagattcaggatcagacacctagtccttccgatggaagcactagacaaagttcacctgagcctaatagtcccagtgaatattgtttttatgt
 
ggatagtgatatggtcaatgagttcaagttggaattggtagaaaaactttttgctgaagacacagaagcaaagaacccattttctactcaggacacagatttagacttggagatgttagctcccta
 
tatcccaatggatgatgacttccagttacgttccttcgatcagttgtcaccattagaaagcagttccgcaagccctgaaagcgcaagtcctcaaagcacagttacagtattccagcagactcaaat
 
acaagaacctactgctaatgccaccactaccactgccaccactgatgaattaaaaacagtgacaaaagaccgtatggaagacattaaaatattgattgcatctccatctcctacccacatacataa
 
agaaactacaagtgccacatcatcaccatatagagatactcaaagtcggacagcctcaccaaacagagcaggaaaaggagtcatagaacagacagaaaaatctcatccaagaagccctaacgtgtt
 
atctgtcgctttgagtcaaagaactacagttcctgaggaagaactaaatccaaagatactagctttgcagaatgctcagagaaagcgaaaaatggaacatgatggttcactttttcaagcagtagg
 
aattggaacattattacagcagccagacgatcatgcagctactacatcactttcttggaaacgtgtaaaaggatgcaaatctagtgaacagaatggaatggagcaaaagacaattattttaatacc
 
ctctgatttagcatgtagactgctggggcaatcaatggatgaaagtggattaccacagctgaccagttatgattgtgaagttaatgctcctatacaaggcagcagaaacctactacagggtgaaga
 
attactcagagctttggatcaagttaacCACCACCACCATCACCACtgagcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatg
 
acagtgttcgaagtttcacgaataaaagataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatgg
 
tagcttcagcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat
 
  
 
===Source===
 
===Source===

Revision as of 20:07, 29 September 2024


HIF1 system for yeast humanisation.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3244
    Illegal BglII site found at 3452
    Illegal BamHI site found at 3355
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 3383
    Illegal AgeI site found at 2972
    Illegal AgeI site found at 4459
  • 1000
    COMPATIBLE WITH RFC[1000]


Source

We utilised several key iGEM BioBricks including: HIF-1 beta and HIF-1 alpha

References