Difference between revisions of "Part:BBa K5117008"
Line 5: Line 5:
 
This part contains the <i>bglB</i> gene of <i>Paenibacillus polymyxa</i>, encoding a β-glucosidase (EC 3.2.1.21).
 
This part contains the <i>bglB</i> gene of <i>Paenibacillus polymyxa</i>, encoding a β-glucosidase (EC 3.2.1.21).
  
−
BsRBS only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page).  
+
PpBglB only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page).  
  
  

Revision as of 20:06, 29 September 2024


PpBglB

This part contains the bglB gene of Paenibacillus polymyxa, encoding a β-glucosidase (EC 3.2.1.21).

PpBglB only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see Contribution page).


Target organism: Bacillus subtilis

Main purpose of use: Gene expression and protein production using the host Bacillus subtilis


Design

For compatibility with the BioBrick RFC[10] standard, the restriction sites EcoRI, XbaI, SpeI, PstI and NotI were removed from the coding sequence. To make the part compatible with the Type IIS standard, BsaI and SapI sites were removed as well. This was achieved by codon exchange using the codon usage table of Bacillus subtilis (Codon Usage Database Kazusa).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]