Difference between revisions of "Part:BBa K5117009"
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<partinfo>BBa_K5117009 short</partinfo>
 
<partinfo>BBa_K5117009 short</partinfo>
  
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bglA gene of Acetivibrio thermocellum, encoding a beta-glucosidase (EC 3.2.1.21)
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This part contains the <i>bglA</i> gene of <i>Acetivibrio thermocellus</i> (synonym Clostridium thermocellum</i>), encoding a β-glucosidase (EC 3.2.1.21).
  
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AtBglA only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page).
  
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<!-- Add more about the biology of this part here <!-- -->
 
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===Usage and Biology===
 
  
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<b>Target organism:</b> <i>Bacillus subtilis</i>
  
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<html>
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<b>Main purpose of use:</b> Gene expression and protein production using the host <i>Bacillus subtilis</i>
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<center><img src="https://static.igem.wiki/teams/5117/parts-registry/subcloning/psb1c3-atbgla.png" style="width: 30%;height: auto;"></center>
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</html>
 
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<p class="image_caption"><center> <h4> Figure 2. </h4> pBS1C3-AtBglA </center></p>
 
  
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===Design===
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For compatibility with the BioBrick RFC[10] standard, the restriction sites <i>Eco</i>RI, <i>Xba</i>I, <i>Spe</i>I, <i>Pst</i>I and <i>Not</i>I were removed from the coding sequence. To make the part compatible with the Type IIS standard, <i>Bsa</i>I and <i>Sap</i>I sites were removed as well. This was achieved by codon exchange using the codon usage table of <i>Bacillus subtilis</i> <html><a href="https://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1423&aa=1&style=N">(Codon Usage Database Kazusa)</a></html>.
  
  

Revision as of 20:06, 29 September 2024


AtBglA

This part contains the bglA gene of Acetivibrio thermocellus (synonym Clostridium thermocellum</i>), encoding a β-glucosidase (EC 3.2.1.21).

AtBglA only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see Contribution page).


Target organism: Bacillus subtilis

Main purpose of use: Gene expression and protein production using the host Bacillus subtilis


Design

For compatibility with the BioBrick RFC[10] standard, the restriction sites EcoRI, XbaI, SpeI, PstI and NotI were removed from the coding sequence. To make the part compatible with the Type IIS standard, BsaI and SapI sites were removed as well. This was achieved by codon exchange using the codon usage table of Bacillus subtilis (Codon Usage Database Kazusa).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]