Difference between revisions of "Part:BBa K5117008"
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<partinfo>BBa_K5117008 short</partinfo> | <partinfo>BBa_K5117008 short</partinfo> | ||
| − | bglB gene of Paenibacillus polymyxa, encoding a | + | This part contains the <i>bglB</i> gene of <i>Paenibacillus polymyxa</i>, encoding a β-glucosidase (EC 3.2.1.21). |
| + | BsRBS only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see <html><a href="https://2024.igem.wiki/tu-dresden/contribution">Contribution</a></html> page). | ||
| − | < | + | |
| − | === | + | <b>Target organism:</b> <i>Bacillus subtilis</i> |
| + | |||
| + | <b>Main purpose of use:</b> Gene expression and protein production using the host <i>Bacillus subtilis</i> | ||
| + | |||
| + | |||
| + | ===Design=== | ||
| + | For compatibility with the BioBrick RFC[10] standard, the restriction sites <i>Eco</i>RI, <i>Xba</i>I, <i>Spe</i>I, <i>Pst</i>I and <i>Not</i>I were removed from the coding sequence. To make the part compatible with the Type IIS standard, <i>Bsa</i>I and <i>Sap</i>I sites were removed as well. This was achieved by codon exchange using the codon usage table of <i>Bacillus subtilis</i> <html><a href="https://www.kazusa.or.jp/codon/cgi-bin/showcodon.cgi?species=1423&aa=1&style=N">(Codon Usage Database Kazusa)</a></html>. | ||
Revision as of 20:05, 29 September 2024
PpBglB
This part contains the bglB gene of Paenibacillus polymyxa, encoding a β-glucosidase (EC 3.2.1.21).
BsRBS only served for design purposes of the TU Dresden iGEM 2024 Team and was required for the construction of composite parts (see Contribution page).
Target organism: Bacillus subtilis
Main purpose of use: Gene expression and protein production using the host Bacillus subtilis
Design
For compatibility with the BioBrick RFC[10] standard, the restriction sites EcoRI, XbaI, SpeI, PstI and NotI were removed from the coding sequence. To make the part compatible with the Type IIS standard, BsaI and SapI sites were removed as well. This was achieved by codon exchange using the codon usage table of Bacillus subtilis (Codon Usage Database Kazusa).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]