Difference between revisions of "Part:BBa K5415004:Design"
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===Source=== | ===Source=== | ||
| − | We utilised | + | We utilised several key iGEM BioBricks including: HIF-1 beta and HIF-1 alpha |
===References=== | ===References=== | ||
Revision as of 20:04, 29 September 2024
HIF1 system for yeast humanisation.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 3244
Illegal BglII site found at 3452
Illegal BamHI site found at 3355 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 929
Illegal NgoMIV site found at 3383
Illegal AgeI site found at 2972
Illegal AgeI site found at 4459 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
ccggtagaggtgtggtcaataagagcgacctcatactatacctgagaaagcaacctgacctacaggaaagagttactcaagaataagaattttcgttttaaaacctaagagtcactttaaaatttg tatacacttattttttttataacttatttaataataaaaatcataaatcataagaaattcgcgctctaGTGGTGATGGTGGTGGTGttctgaaaaggggggaaacatagttagatcaggaaattct tcattgttgtagctgttgctctgatctcccagcatggacagcatctcctggaagacctcaggctggccaggttgctgtgctggcggttgttgaacatgttgctcactagaacttgaacgatgatga ggctgctggccctgccactgtggccagacacccacaccctctgctgtccgtgtctggaattgtccggcagtctgtccagtctcaggagcaaagttagatccacgattggtgagactagggtaggca gcagcaccaggcgatgcagttggggcaccagggagggacatggagctgaaggaggatggagtctgaaagctgcccacaccaaactgggaagtacgagtcttagcagtagcctgggtagccacctgc tgggcagaaaagcctgagcgggtagtaggggtccaagttggggttgctccttgggtggggttggagtggcgggaaatctgggccaacatctgtcccgcagaagctgatggctggacaatggttaca ggaggggctaggccactattcctgaaattctctgccggccggggggtaggagggaatgtgttgccctgggagaatagctgttgggtggcagggacagtgctggaggagatgcctttactctgatcc gcattgatgttgtgatagatttctgaaaatcttggatctctatcctgggcaaataaaccatctgacttctcaaggggcttgctgtgttctggtcctgtggttgtcacaggctgaaccacctgggaa tgattgtagctggccagtccatctcttcctggtaccatgtccaattctgtttgctgttgctgctgcctgggtgccagctgtcctgagcccatctccaggggtaaattagctgtgggacctagttgt ggcctctggattgtgttggagagtgtaggccgtggttcttggctagagttcttcacattggtgttggtacagatgatgtactcaatttcatctgagtaagggttctggaaagtaaaggagctggtt ctcatccagagccattcttggttcttagaccggaaccggaacatgacagacagcacttggccttttaatttcactacctgttggaagctgtctcttagaagctgctggtcttcaggatgacaaaat tctacaatattctttcctaagagttcctgtggctggtagccaacagtagccacacagcggtgatccacaaaagtgaagataccctcaatgttgtgtcgggagatgaactctgttggttgacaaaca ttactcatgtctgtacagttgggagaactggttacctgcaatctgccaatggccactaggcaaaacttgcttccctggccagcctctgggtcatcatctgggagggaaacacctgctgggggccag gccctgatgtagcctgtgcagtggaccaccacgaagtgaggttccccatcctttacagagccaagtccattcctgcatctgttcctcacaaagctcagcctattcacagaaactgggtccacagag ctactgccacacctcattcggcaaataaacgatctccttgagcccatacacattctcatggaagactgctgaccttcctttttcactgttccagtctttagatccaggatacgccctgtcagggca ttttctgaagtggaaagctgctcacgaagtttatccacatcatctgggtgcacctgatcatagagtgtgctgccaaaccattcagactgtggctggttcaaaacaggagtcacggagtcagacaca tacaccaccctgcctgtctcacatgagacaataaacagaaagccatctgctgcctccaagatcaaatgtttcagttcctgatcagtgaggaaagacggcttataggagccatcagtggatgtgttg ccagttccccgcaaggacttcatgtgagaaactgccatgcgtaagatggttagcttgtctggttttcgagccagggcactacaggtgggtaccatatctgacagttctgtgatgtaggctgtcatc ttgttccgtcgccgccgttcaatttcactgtgattttccctggcaaaccgctccttatcgttagacatctgatcatcatcacacctcaaaaatttactgttcccttctccatcatcatcaaaatcc agccctggtcgccgcttaatagccctctggacaatggctcctccaccttggattccaggtccagagtttccagaggcaatggctggacccagtgatggtacatctgatgtcatttcggggttggca gtagtcgccgccatcgaattttcaaaaattcttactttttttttggatggacgcaaagaagtttaataatcatattacatgtgtgaaattgttatccgctcacaattccacacaacatatctaatc ttacttatatgttgtggaaatgtaaagagccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccg agcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactag cttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaat taatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgtccctatcagtgatagagatctccctatcagtgatagagatattacttcttattcaaatgtaataaaagt atcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaaccccggatcctattaaaatggagggcgccggcggcgcgaacgacaagaaaaagataagttctgaacgtcgaaa agaaaagtctcgtgatgcagccagatctcggcgaagtaaagaatctgaagttttttatgagcttgctcatcagttgccacttccacataatgtgagttcgcatcttgataaggcctctgtgatgag gcttaccatcagctatttgcgtgtgaggaaacttctggatgctggtgatttggatattgaagatgacatgaaagcacagatgaattgcttttatttgaaagccttggatggttttgttatggttct cacagatgatggtgacatgatttacatttctgataatgtgaacaaatacatgggattaactcagtttgaactaactggacacagtgtgtttgattttactcatccatgtgaccatgaggaaatgag agaaatgcttacacacagaaatggccttgtgaaaaagggtaaagaacaaaacacacagcgaagtttttttctcagaatgaagtgtaccctaactagccgaggaagaactatgaacataaagtctgc aacatggaaggtattgcactgcacaggccacattcacgtatatgataccaacagtaaccaacctcagtgtgggtataagaaaccacctatgacctgcttggtgctgatttgtgaacccattcctca cccatcaaatattgaaattcctttagatagcaagactttcctcagtcgacacagcctggatatgaaattttcttattgtgatgaaagaattaccgaattgatgggatatgagccagaagaactttt aggccgctcaatttatgaatattatcatgctttggactctgatcatctgaccaaaactcatcatgatatgtttactaaaggacaagtcaccacaggacagtacaggatgcttgccaaaagaggtgg atatgtctgggttgaaactcaagcaactgtcatatataacaccaagaactctcaaccacagtgcattgtatgtgtgaattacgttgtgagtggtattattcagcacgacttgattttctcccttca acaaacagaatgtgtccttaaaccggttgaatcttcagatatgaaaatgactcagctattcaccaaagttgaatcagaagatacaagtagcctctttgacaaactcaagaaggaacctgatgcttt aactttgctggccccagccgctggagacacaatcatatctttagattttggcagcaacgacacagaaactgatgaccagcaacttgaggaagtaccattatataatgatgtaatgctcccctcacc caacgaaaaattacagaatataaatttggcaatgtctccattacccaccgctgaaacgccaaagccacttcgaagtagtgctgaccctgcactcaatcaagaagttgcattaaaattagaaccaaa tccagagtcactggaactttcttttaccatgccccagattcaggatcagacacctagtccttccgatggaagcactagacaaagttcacctgagcctaatagtcccagtgaatattgtttttatgt ggatagtgatatggtcaatgagttcaagttggaattggtagaaaaactttttgctgaagacacagaagcaaagaacccattttctactcaggacacagatttagacttggagatgttagctcccta tatcccaatggatgatgacttccagttacgttccttcgatcagttgtcaccattagaaagcagttccgcaagccctgaaagcgcaagtcctcaaagcacagttacagtattccagcagactcaaat acaagaacctactgctaatgccaccactaccactgccaccactgatgaattaaaaacagtgacaaaagaccgtatggaagacattaaaatattgattgcatctccatctcctacccacatacataa agaaactacaagtgccacatcatcaccatatagagatactcaaagtcggacagcctcaccaaacagagcaggaaaaggagtcatagaacagacagaaaaatctcatccaagaagccctaacgtgtt atctgtcgctttgagtcaaagaactacagttcctgaggaagaactaaatccaaagatactagctttgcagaatgctcagagaaagcgaaaaatggaacatgatggttcactttttcaagcagtagg aattggaacattattacagcagccagacgatcatgcagctactacatcactttcttggaaacgtgtaaaaggatgcaaatctagtgaacagaatggaatggagcaaaagacaattattttaatacc ctctgatttagcatgtagactgctggggcaatcaatggatgaaagtggattaccacagctgaccagttatgattgtgaagttaatgctcctatacaaggcagcagaaacctactacagggtgaaga attactcagagctttggatcaagttaacCACCACCACCATCACCACtgagcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatg acagtgttcgaagtttcacgaataaaagataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtagacatgg tagcttcagcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat
Source
We utilised several key iGEM BioBricks including: HIF-1 beta and HIF-1 alpha