Difference between revisions of "Part:BBa K5108003"
Yohannquivet (Talk | contribs) |
Yohannquivet (Talk | contribs) |
||
| Line 22: | Line 22: | ||
<p><a href="https://www.uniprot.org/uniprotkb/P38488/entry" target="blank">Creatinase</a> (CreA) is an enzyme that catalyzes the degradation of creatine into sarcosine in <i>Pseudomonas putida</i>. | <p><a href="https://www.uniprot.org/uniprotkb/P38488/entry" target="blank">Creatinase</a> (CreA) is an enzyme that catalyzes the degradation of creatine into sarcosine in <i>Pseudomonas putida</i>. | ||
| − | In the context of our project, the gene encoding this enzyme (<i>creA</i>) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct and our results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a>. | + | In the context of our project, the gene encoding this enzyme (<i>creA</i>) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct and our results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a>. Figure 1 displays the intermediate step of construction. |
</p> | </p> | ||
| − | + | <br> | |
| − | + | <div align="center"> | |
| + | <figure class="normal mx-auto"> | ||
| + | <img class="d-block" | ||
| + | style="width:100%;" | ||
| + | src="https://static.igem.wiki/teams/5108/lea/crea-part.png"><br><br> | ||
| + | <figcaption class="normal"><span class="titre-image"><b>Figure 1: Design of the expression plasmid harboring the <i>creA</i> gene.</b></span></figcaption> | ||
| + | </figure> | ||
| + | </div> | ||
| + | <br> | ||
<br> | <br> | ||
<h2 style="color: blue;"><b>References</b></h2> | <h2 style="color: blue;"><b>References</b></h2> | ||
Revision as of 19:26, 29 September 2024
Creatinase from Pseudomonas putida
Creatinase from Pseudomonas putida
- Contents
- Usage and Biology
- References
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 225
Illegal BsaI site found at 1012
Usage and Biology
Creatinase (CreA) is an enzyme that catalyzes the degradation of creatine into sarcosine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (creA) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct and our results in BBa_K5108009. Figure 1 displays the intermediate step of construction.
