Difference between revisions of "Part:BBa K5415003"

 
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<partinfo>BBa_K5415003 short</partinfo>
 
<partinfo>BBa_K5415003 short</partinfo>
  
The composite part we have developed is a hypoxia biosensor designed to detect low hypoxic conditions, built in silico using Benchling and HRE gene sequences imported from the https://doi.org/10.1016/j.bbrc.2006.06.043, pMC3-yeGFP imported from Addgene (Plasmid #176181). Gibson and in vivo cloning methods were employed. The constructs were transformed into E. coli.
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The composite part we have developed is a hypoxia biosensor designed to detect low hypoxic conditions, built <i>in silico</i> using Benchling and HRE gene sequences imported from the https://doi.org/10.1016/j.bbrc.2006.06.043, pMC3-yeGFP imported from <i>Addgene</i> (Plasmid #176181). Gibson and <i>in vivo</i> cloning methods were employed. The constructs were transformed into <i>E. coli</i>.
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The biosensor construct features three key components: nine hypoxia response elements (HREs), restriction sites (NcoI), and a red fluorescent protein (RFP). The hypoxia response elements are short DNA sequences that respond to the binding of hypoxia-inducible factor (HIF) complexes, which are activated in low oxygen environments. These binded HRE sequences trigger the expression of the RFP, allowing the cell to fluoresce under hypoxic conditions. The use of two restriction sites, NcoI, facilitates further manipulation of the construct if needed.  
 
The biosensor construct features three key components: nine hypoxia response elements (HREs), restriction sites (NcoI), and a red fluorescent protein (RFP). The hypoxia response elements are short DNA sequences that respond to the binding of hypoxia-inducible factor (HIF) complexes, which are activated in low oxygen environments. These binded HRE sequences trigger the expression of the RFP, allowing the cell to fluoresce under hypoxic conditions. The use of two restriction sites, NcoI, facilitates further manipulation of the construct if needed.  
  

Revision as of 18:23, 29 September 2024


Nonuple HRE biosensor with RFP for severe hypoxia detection.

The composite part we have developed is a hypoxia biosensor designed to detect low hypoxic conditions, built in silico using Benchling and HRE gene sequences imported from the https://doi.org/10.1016/j.bbrc.2006.06.043, pMC3-yeGFP imported from Addgene (Plasmid #176181). Gibson and in vivo cloning methods were employed. The constructs were transformed into E. coli.

The biosensor construct features three key components: nine hypoxia response elements (HREs), restriction sites (NcoI), and a red fluorescent protein (RFP). The hypoxia response elements are short DNA sequences that respond to the binding of hypoxia-inducible factor (HIF) complexes, which are activated in low oxygen environments. These binded HRE sequences trigger the expression of the RFP, allowing the cell to fluoresce under hypoxic conditions. The use of two restriction sites, NcoI, facilitates further manipulation of the construct if needed.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1
    Illegal XhoI site found at 21
    Illegal XhoI site found at 35
    Illegal XhoI site found at 55
    Illegal XhoI site found at 69
    Illegal XhoI site found at 89
    Illegal XhoI site found at 103
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]