Difference between revisions of "Part:BBa K5237015"
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<partinfo>BBa_K5237327 short</partinfo> | <partinfo>BBa_K5237327 short</partinfo> | ||
| − | This part can be used for the display of nanobodies on the surface of <i>E.coli</i>. Salema, V. et al, (2013) showed efficient presentation of nanobodies on the surface of <i>E.coli</i> K-12 cells by fusing them to the β domain of Intimin. The part comes from the pNeae2 plasmid (addgene #168300) and encodes an N-terminal signal peptide for Sec-dependent translocation into the periplasm, a LysM domain that interacts with the peptidoglycan and provides anchoring, and a β-barrel that inserts into the outer membrane. The C-terminus is exposed to the extracellular milieu and contains a myc tag that can be used for purification or detection of full-length protein. | + | This part can be used for the display of nanobodies on the surface of <i>E.coli</i>. Salema, V. et al, (2013) showed efficient presentation of nanobodies on the surface of <i>E.coli</i> K-12 cells by fusing them to the β domain of Intimin. The part comes from the pNeae2 plasmid (addgene #168300) and encodes an N-terminal signal peptide for Sec-dependent translocation into the periplasm, a LysM domain that interacts with the peptidoglycan and provides anchoring, and a β-barrel that inserts into the outer membrane. The C-terminus is exposed to the extracellular milieu and contains a myc tag (EQKLISEED) that can be used for purification or detection of full-length protein. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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| − | + | Uncomment this to enable Functional Parameter display | |
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K5237327 parameters</partinfo> | <partinfo>BBa_K5237327 parameters</partinfo> | ||
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Revision as of 18:18, 29 September 2024
No part name specified with partinfo tag.
This part can be used for the display of nanobodies on the surface of E.coli. Salema, V. et al, (2013) showed efficient presentation of nanobodies on the surface of E.coli K-12 cells by fusing them to the β domain of Intimin. The part comes from the pNeae2 plasmid (addgene #168300) and encodes an N-terminal signal peptide for Sec-dependent translocation into the periplasm, a LysM domain that interacts with the peptidoglycan and provides anchoring, and a β-barrel that inserts into the outer membrane. The C-terminus is exposed to the extracellular milieu and contains a myc tag (EQKLISEED) that can be used for purification or detection of full-length protein.
Sequence and Features No part name specified with partinfo tag.
Uncomment this to enable Functional Parameter display
Functional Parameters
No part name specified with partinfo tag.