Difference between revisions of "Part:BBa K243037"
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| − | The protein expression starts only when induced by arabinose, so this vector is useful for the expression of toxic products. | + | The protein expression starts only when induced by arabinose, so this vector is useful for the expression of toxic products.<br> |
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| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'>'''Sequence and Features'''</span> |
<partinfo>BBa_K243037 SequenceAndFeatures</partinfo> | <partinfo>BBa_K243037 SequenceAndFeatures</partinfo> | ||
Revision as of 22:27, 21 October 2009
pBAD
The cloning vector pBAD has the RFC25 restriction sites and an ampicilin resistance. It is inducible by arabinose and when the inserted part has an RBS, it can be used as an expression vector.
plasMap
Usage and Biology
The protein expression starts only when induced by arabinose, so this vector is useful for the expression of toxic products.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 2434
Illegal suffix found in sequence at 3051 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2434
Illegal SpeI site found at 3052
Illegal PstI site found at 3066
Illegal NotI site found at 2440
Illegal NotI site found at 3059 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2434
Illegal BamHI site found at 2338 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2434
Illegal suffix found in sequence at 3052 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2434
Illegal suffix found in sequence at 3042 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3106
Illegal BsaI site found at 3284
Illegal BsaI site found at 4352
Illegal SapI site found at 2155
Illegal SapI.rc site found at 783
Illegal SapI.rc site found at 2949