Difference between revisions of "Part:BBa K5127006:Design"
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===Design Notes=== | ===Design Notes=== | ||
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli. | This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli. | ||
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===Source=== | ===Source=== | ||
Revision as of 17:55, 29 September 2024
iadCDE
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2355
Illegal PstI site found at 2172
Illegal PstI site found at 2750 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2355
Illegal NheI site found at 677
Illegal PstI site found at 2172
Illegal PstI site found at 2750 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2355
Illegal BglII site found at 2364
Illegal BglII site found at 2416 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 2355
Illegal PstI site found at 2172
Illegal PstI site found at 2750 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 2355
Illegal PstI site found at 2172
Illegal PstI site found at 2750
Illegal NgoMIV site found at 623
Illegal NgoMIV site found at 1614
Illegal AgeI site found at 387
Illegal AgeI site found at 1416
Illegal AgeI site found at 1857
Illegal AgeI site found at 2280
Illegal AgeI site found at 2714 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is codon optimized for Goldengate assembly (BsaI, BsmBI, BbsI) and expression in E.coli.
Source
Genome of Variovorax species
References
Conway, J. M., Walton, W. G., Salas-González, I., Law, T. F., Lindberg, C. A., Crook, L. E., Kosina, S. M., Fitzpatrick, C. R., Lietzan, A. D., Northen, T. R., Jones, C. D., Finkel, O. M., Redinbo, M. R., & Dangl, J. L. (2022). Diverse MarR bacterial regulators of auxin catabolism in the plant microbiome. Nature Microbiology, 7(11), 1817–1833. https://doi.org/10.1038/s41564-022-01244-3