Difference between revisions of "Part:BBa K5035005:Design"

 
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A0A2E5NSL8 is a corrinoid iron-sulfur-containing reductive dehalogenase produced by the Dehalococcoidia bacterium. A0A2E5NSL8 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Because of A0A2E5NSL8's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities.
  
__NOTOC__
 
<partinfo>BBa_K5035005 short</partinfo>
 
 
<partinfo>BBa_K5035005 SequenceAndFeatures</partinfo>
 
 
 
===Design Notes===
 
 
A hexahistidine tag for nickel column purification was added to A0A2E5NSL8.
 
A hexahistidine tag for nickel column purification was added to A0A2E5NSL8.
  
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References:
  
 
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https://www.uniprot.org/uniprotkb/A0A2E5NSL8/publications
===Source===
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ATGCATCATCATCATCATCATATCCATCCGCGTGGCTCCGAACGTCCAGACCGTCGTGGCGCTCTGTTCGCTGGTTCTCCGTCCGATAGCTGTGACGCGGGTCCTTCTGGCGTAGACGGCCCAGCTTGGGAAGTTCTGGAAAGCTTTGAACGCTTCAACCAGGTTGACGACGTATTCAACCGTGCTAGCTGGGACCCAGAAGTGGCAACCGAGGAAGCTCGCCGCTTTTTTGCGTCTTATCGTGAACCGCTGCGCTCCTGGCGTAACGCAAGCGGCTTCGGCCAGCGTGATTATGCAATTCGCAACGCCGCTTGGCAAGTTGTAGATGTCTTCGCAGAGATGCACGACGCTGAAGATCTGCGTGACGGTTTTCTGAGCCCGCTGTCTCAACTGCGTGATGGCCCGTCTGAACGCTTTGATCTGGGTTCTCCGGAAGAAGCTTCTGCTACGGTGAAACAGGTTGCACGTACCTTCGGCGCTGACCTGGTTGGTCTGTGCGCGCACGATGAACGCTGGGTGTACACCGAACGTTACGCGCGTCGTGCGGATGAAGGTCGTCCAGTCGGCCTGCCGGATGGTCTGACCAGCGTGGTTGTCATTGGTCAGGCTATGGACCGTGGCCTGGTTGATACCGTACCGTCCGCACTGTCCGGTGCCGCTACCGGTCTGGGTTACTCCAAAGACGCTCTGGTCCTGCTGGCAACGGCACAGTGGCTGCGTAACCTGGGTTATCGCGCAGTTCCTTCTCTGAACGACACGGCGCTGGCTATCCCGCTGGCAATTCAAGCAGGTCTGGGTGAATACGGCCGCCACGGCATGGTAATCACCCCGGAATTTGGCCCGCGTGTACGTTTCGGCAAAATCTTCACCGATCTGCCTGTGGCCCACGACCAGCCTATCGAATTCGGTGTTACTCGCTTCTGCGAGCAGTGTCGCCGTTGCTCCAACGCATGCCCGCCAGGTGCAATTCCGGACGCAGGCCCGGTGGCGGTAAAACTGAACCGCAGCAGCATCGGTGGCGTTCGTAAGTGGTCCGTGGACGGCGAAAAATGCTTCAGCTACTGGGCTCAGATCGCGAACGACTGCATGATCTGTATGCGCGTCTGTCCGTACAACAAAGACTTTTCCCGTTTCCGTCACCGTCTGGGTCGTCGTCTGGCTGCTACGCGTCTGCGCGGTATGATGCTGTGGCTGGAAGAACGTATGGGTTACGGCAAGCGCCTGCGTCCACGTGACTGGTGGAAAGGTCGTGACGATCGTCCGACCCTACTCTGGCGTCGATGA
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===References===
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Revision as of 17:41, 29 September 2024

A0A2E5NSL8 is a corrinoid iron-sulfur-containing reductive dehalogenase produced by the Dehalococcoidia bacterium. A0A2E5NSL8 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Because of A0A2E5NSL8's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities.

A hexahistidine tag for nickel column purification was added to A0A2E5NSL8.

References:

https://www.uniprot.org/uniprotkb/A0A2E5NSL8/publications