Difference between revisions of "Part:BBa K243035"

Revision as of 22:21, 21 October 2009

pJS419

The pJS419 vector is used as an expression vector. It has a weak Shine-Dalgano sequence that enables low expression level of the protein, which can be useful for proteins with a complex stucture or even toxic proteins.

Usage and Biology

The vector is used for expression of complex molecules in order not to overburden the expressing cells. Also, it is used for proteins that tend to aggregate as well as for the expression of cell toxic proteins.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal suffix found in sequence at 2266
Illegal XbaI site found at 1382
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 2267
Illegal PstI site found at 2281
Illegal NotI site found at 2274
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1494
Illegal BamHI site found at 2365
23
INCOMPATIBLE WITH RFC[23]
Illegal suffix found in sequence at 2267
Illegal XbaI site found at 1382
25
INCOMPATIBLE WITH RFC[25]
Illegal suffix found in sequence at 2257
Illegal XbaI site found at 1382
Illegal NgoMIV site found at 1390
Illegal NgoMIV site found at 2498
Illegal AgeI site found at 3832
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2114

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-The vector is used for expression of complex molecules in order not to overburden the expressing cells. Also, it is used for proteins that tend to aggregate as well as for the expression of cell toxic proteins.
+The vector is used for expression of complex molecules in order not to overburden the expressing cells. Also, it is used for proteins that tend to aggregate as well as for the expression of cell toxic proteins.<br>
 <span class='h3bb'>'''Sequence and Features'''</span>
 <partinfo>BBa_K243035 SequenceAndFeatures</partinfo>