Difference between revisions of "Part:BBa K5109021"
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<partinfo>BBa_K5109021 short</partinfo> | <partinfo>BBa_K5109021 short</partinfo> | ||
| − | This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with | + | This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with Laccase E (BBa_K5109014). This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme. To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence. |
===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 15:42, 29 September 2024
Laccase E surface display system
This composite part is identical to BBa_K5109023, except that the gene for the desired surface expression has been substituted with Laccase E (BBa_K5109014). This modification can be achieved using cloning techniques, specifically by digesting the surface expression cassette BBa_K5109023 with BsaI and BamHI, followed by ligation with the desired enzyme. To do this, specific BsaI and BamHI restriction sites must be added to the enzyme’s sequence.
Usage and Biology
This surface display system aims to express the laccase on the outer side of the cell membrane. This way, we could test it's functioning on PFAS molecules directly on the external medium, without stressing or damaging E. coli by expressing the enzyme inside.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 639
Illegal BamHI site found at 2245 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 900
Illegal NgoMIV site found at 1121 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 667