Difference between revisions of "Part:BBa K243032:Design"
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The combination of the parts [https://parts.igem.org/Part:BBa_243000 Fok_a] and [https://parts.igem.org/Part:BBa_I757008 mVenus-YFP] was done to gain information about protein expression. The mVenusYFP is a proven Biobrick of the [http://parts.mit.edu/igem07/index.php/Freiburg team Freiburg07]. Thus, Venus fusion proteins give an easy to visualize readout of protein expression and are also good detectable by western blotting. | The combination of the parts [https://parts.igem.org/Part:BBa_243000 Fok_a] and [https://parts.igem.org/Part:BBa_I757008 mVenus-YFP] was done to gain information about protein expression. The mVenusYFP is a proven Biobrick of the [http://parts.mit.edu/igem07/index.php/Freiburg team Freiburg07]. Thus, Venus fusion proteins give an easy to visualize readout of protein expression and are also good detectable by western blotting. | ||
| − | Designed according the [https://parts.igem.org/Assembly_standard_25 RFC 25]. | + | Designed according to the [https://parts.igem.org/Assembly_standard_25 RFC 25]. |
===Source=== | ===Source=== | ||
Revision as of 22:16, 21 October 2009
Fok_a-Venus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 487
Design Notes
The combination of the parts Fok_a and mVenus-YFP was done to gain information about protein expression. The mVenusYFP is a proven Biobrick of the [http://parts.mit.edu/igem07/index.php/Freiburg team Freiburg07]. Thus, Venus fusion proteins give an easy to visualize readout of protein expression and are also good detectable by western blotting.
Designed according to the RFC 25.
Source
Combined the parts by serial cloning steps.