Difference between revisions of "Part:BBa K5382140:Experience"
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===Applications of BBa_K5382140=== | ===Applications of BBa_K5382140=== | ||
| − | Extrudable outer membrane vesicles containing Cas9 RNP were delivered into HeLa cells, and the gene-editing activity of prepared OMV-Cas9 RNP was compared with commercial liposomal transfection reagents by T7E1 assay (FIG. 1). | + | Extrudable outer membrane vesicles containing Cas9 RNP were delivered into HeLa cells, and the gene-editing activity of prepared OMV-Cas9 RNP was compared with commercial liposomal transfection reagents by T7E1 assay (FIG. 1).<br> |
| − | Figure 1. Verification experiment of genome editing activity of | + | https://static.igem.wiki/teams/5382/part-pictures/gene-editing1.png<br>'''Figure 1.'''Verification experiment of genome editing activity of Hela cells.<br> |
The results of cutting the target gene showed that the prepared OMV-Cas9 RNP had similar genome editing activity to the commercial liposome transfection reagent, both of which were about 40%. | The results of cutting the target gene showed that the prepared OMV-Cas9 RNP had similar genome editing activity to the commercial liposome transfection reagent, both of which were about 40%. | ||
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Applications of BBa_K5382140
Extrudable outer membrane vesicles containing Cas9 RNP were delivered into HeLa cells, and the gene-editing activity of prepared OMV-Cas9 RNP was compared with commercial liposomal transfection reagents by T7E1 assay (FIG. 1).
Figure 1.Verification experiment of genome editing activity of Hela cells.
The results of cutting the target gene showed that the prepared OMV-Cas9 RNP had similar genome editing activity to the commercial liposome transfection reagent, both of which were about 40%.
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