Difference between revisions of "Part:BBa K5366050"

 
 
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<partinfo>BBa_K5366050 short</partinfo>
 
<partinfo>BBa_K5366050 short</partinfo>
  
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During the experiment, it was observed that the biosensor responded to the target substance by transporting tagatose from the environment into the cells. Escherichia coli Nissle 1917 (EcN) has been demonstrated to utilize tagatose as a carbon source due to the presence of a series of gene clusters related to tagatose metabolism, including tagatose transporters. These gene clusters play a crucial role, as they not only encode specific transporters that effectively recognize and transport tagatose, but also include various enzymatic genes that enable the rapid breakdown and utilization of tagatose once it is inside the cells. This process significantly enhances the absorption efficiency of tagatose, allowing E.coli Nissle 1917 to grow and replicate swiftly in a tagatose-rich environment.
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During the experiment, it was observed that the biosensor responded to the target substance by transporting tagatose from the environment into the cells. <i>Escherichia coli</i> Nissle 1917 (EcN) has been demonstrated to utilize tagatose as a carbon source due to the presence of a series of gene clusters related to tagatose metabolism, including tagatose transporters. These gene clusters play a crucial role, as they not only encode specific transporters that effectively recognize and transport tagatose, but also include various enzymatic genes that enable the rapid breakdown and utilization of tagatose once it is inside the cells. This process significantly enhances the absorption efficiency of tagatose, allowing <i>E.coli Nissle</i> 1917 to grow and replicate swiftly in a tagatose-rich environment.
  
 
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Latest revision as of 14:20, 29 September 2024


Gene Cluster

During the experiment, it was observed that the biosensor responded to the target substance by transporting tagatose from the environment into the cells. Escherichia coli Nissle 1917 (EcN) has been demonstrated to utilize tagatose as a carbon source due to the presence of a series of gene clusters related to tagatose metabolism, including tagatose transporters. These gene clusters play a crucial role, as they not only encode specific transporters that effectively recognize and transport tagatose, but also include various enzymatic genes that enable the rapid breakdown and utilization of tagatose once it is inside the cells. This process significantly enhances the absorption efficiency of tagatose, allowing E.coli Nissle 1917 to grow and replicate swiftly in a tagatose-rich environment.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3646
    Illegal EcoRI site found at 4094
    Illegal PstI site found at 479
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3646
    Illegal EcoRI site found at 4094
    Illegal PstI site found at 479
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3646
    Illegal EcoRI site found at 4094
    Illegal BamHI site found at 290
    Illegal BamHI site found at 3733
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3646
    Illegal EcoRI site found at 4094
    Illegal PstI site found at 479
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3646
    Illegal EcoRI site found at 4094
    Illegal PstI site found at 479
    Illegal AgeI site found at 1730
    Illegal AgeI site found at 4617
    Illegal AgeI site found at 5112
  • 1000
    COMPATIBLE WITH RFC[1000]