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Creatinase from <i>Pseudomonas putida</i>
 
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<h2 style="color: blue;"> <b>Usage and Biology</b></h2>
 
<h2 style="color: blue;"> <b>Usage and Biology</b></h2>
  
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<p><a href="https://www.uniprot.org/uniprotkb/P38488/entry" target="blank">CreA</a> is an enzyme that catalyzes the degradation of creatine into sarcosine in <i>Pseudomonas putida</i>.  
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<p><a href="https://www.uniprot.org/uniprotkb/P38488/entry" target="blank">Creatinase</a> (CreA) is an enzyme that catalyzes the degradation of creatine into sarcosine in <i>Pseudomonas putida</i>.  
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In the context of our project, the gene encoding this enzyme (<i>creA</i>) was synthesized in an operon with the gene for creatine degradation. You can find more information about our construct and our results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a>.  
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In the context of our project, the gene encoding this enzyme (<i>creA</i>) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct and our results in <a href="https://parts.igem.org/Part:BBa_K5108009" target="blank">BBa_K5108009</a>.  
 
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Revision as of 13:57, 29 September 2024

Creatinase from Pseudomonas putida

Creatinase from Pseudomonas putida

    Contents
  1. Usage and Biology
  2. References



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 225
    Illegal BsaI site found at 1012

Usage and Biology

Creatinase (CreA) is an enzyme that catalyzes the degradation of creatine into sarcosine in Pseudomonas putida. In the context of our project, the gene encoding this enzyme (creA) was synthesized in an operon together with the gene for creatine degradation. You can find more information about construct and our results in BBa_K5108009.


References

  1. UniProt. (s. d.-b). https://www.uniprot.org/uniprotkb/P38488/entry
  2. Cloning, Expression and Purification of Pseudomonas putida ATCC12633 Creatinase. (2017, 1 décembre). PubMed. https://pubmed.ncbi.nlm.nih.gov/29090065/
    3.