Difference between revisions of "Part:BBa K211002:Design"
(→Design Notes) |
(→Design Notes) |
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Designed with PCR. | Designed with PCR. | ||
[[Image:primer design.jpg]] | [[Image:primer design.jpg]] | ||
− | Primer Sequence | + | Primer Sequence |
P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC | P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC | ||
Line 20: | Line 20: | ||
P5 Odr-10 R GCAGTA AATGATCAGAATCAGAATGAG | P5 Odr-10 R GCAGTA AATGATCAGAATCAGAATGAG | ||
− | P6 RI7-C F GATCATT TACTGCTTGCGCAACC | + | P6 RI7-C F GATCATT TACTGCTTGCGCAACC |
− | BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC | + | BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC |
− | BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC | + | BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC |
Revision as of 22:08, 21 October 2009
RI7-odr10 chimeric GPCR
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Designed with PCR. Primer Sequence P1 RI7-N F ATATCTGAATTCGCCACCATGGAACGAAGGAACCAC
P2 RI7-C R GTATACGCGGCCGCACCAATTTTGCTGCCTTTG
P3 RI7-N R GAGAAGAA ATACATGGGTTTGTGGAG
P4 Odr-10 F CCATGTAT TTCTTCTCAGTATTCTCG
P5 Odr-10 R GCAGTA AATGATCAGAATCAGAATGAG
P6 RI7-C F GATCATT TACTGCTTGCGCAACC
BioBrick F ATATCT TCTAGATGGAACGAAGGAACCAC
BioBrick R GTATACACTAGTA TTA TTA ACCAATTTTGCTGCC
We would like to thank Professor Piali Sengupta, Department of Biology, Brandeis University for the kind gift of the odr-10 cDNA, and Professor Stuart Firestein, Department of Biological Sciences, Columbia University for the kind gift of the rat I7 olfactory receptor cDNA.
Source
Caenorhabditis elegans olfactory receptor Odr-10 (GenBank: U49449.1)
Rat olfactory protein, clone I7 (GenBank: M64386.1)
References
Radhika, V. et al . 2007. Chemical sensing of DNT by engineered olfactory yeast strain. Nat. Chem. Biol. 3 : 325–330